figure3c: Monitoring growth of miR34a asRNA overexpressings cells under...

In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.

Description

10^4 PC3 cells, either miR34a asRNA overexpressing or mock, were seeded in 96 well plates. After attachment (3-4h) media was replaced with either RPMI (Gibco, life technology) (supplemented with 2mM L-glutamine, 50ug/ml Penicillin-Streptomycin and 10 incubated in Spark Multimode Microplate reader for 48 hours at 37<c2><b0>C with 5 CO2 in a humidity chamber. Confluency was measured every hour. Three technical triplicates were included in each experiment and these were independantly normalized for each condition to the 0 time point afterwhich the mean was calculated. The 95 based on the three independant experiments which were preformed.

Format

A tibble with 1296 rows and 6 variables:

'Cell line'

Factor; Indicates the cell line the experiment was performed in.

Treatment

Factor; Indicates the cell line the experiment was performed in.

'Biological Replicate'

Numeric; Indicates the biological replicate of the sample.

'Technical Replicate'

Factor; indicates the technical replicate.

Time

Character; Time in hours after treatment.

Confluency

Numeric; The percentage of confluency.

...

Examples

getData('Figure 3c')

GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.