figure3d: PC3 stable line pollII ChIP.

In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.

Description

QPCR was performed in technical replicate for each sample and the mean of the technical replicates was subsequently used to calculate the fraction of input. The fold change of the miR34a asRNA overexpression samples was then calculated by dividing the values by the values for the corresponding mock samples. The fold values were then log2 transformed and the Student's t-test was used to test for significant differences under the null hypothesis that the true mean value of log2 transformed fold changes were equal to 0.

Format

A tibble with 16 rows and 7 variables:

'Biological Replicate'

Numeric; Indicates the biological replicate of the sample.

'Cell line'

Factor; Indicates the cell line the experiment was performed in.

condition

Factor; Indicates the genetic modification in the cell.

sample

Factor; indicates the sample type.

gene

Character; Gene amplified in QPCR.

Quantity1

Numeric; The absolute RNA value from technical replicate 1.

Quantity2

Numeric; The absolute RNA value from technical replicate 2.

...

Examples

getData('Figure 3d')

GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.