supp_figure4c: CCND1 expression in PC3 stable cell lines.

In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.

Description

All cell lines were cultured at 5 (Hyclone) and 2 mM L-glutamine. All growth mediums were supplemented with 10 penicillin. Cells were seeded at 2x10^5 cells per well in a 12-well plate. After 24 hours, RNA was extracted using the RNeasy mini kit (Qiagen) and subsequently treated with DNase (Ambion Turbo DNA-free, Life Technologies). 500ng RNA was used for cDNA synthesis using MuMLV (Life Technologies) and a 1:1 mix of oligo(dT) and random nanomers. QPCR was carried out using KAPA 2G SYBRGreen (Kapa Biosystems) using the Applied Biosystems 7900HT machine with the cycling conditions: 95 <c2><b0>C for 3 min, 95 <c2><b0>C for 3 s, 60 <c2><b0>C for 30 s.

Format

A tibble with 24 rows and 6 variables:

'Biological Replicate'

Numeric; Indicates the biological replicate of the sample.

'Cell line'

Character; indicates the cell line the experiment was performed in.

condition

Factor; Indicates the genetic modifications in the cell line.

gene

Character; indicates the gene that the Ct values correspond to.

Ct1

Numeric; Ct value corresponding to the first technical replicate.

Ct2

Numeric; Ct value corresponding to the 2nd technical replicate.

...

Examples

getData('Supplementary Figure 4c')

GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.