figure3b: miR34a asRNA PC3 stable cell line cell cycle.

In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.

Description

All cell lines were cultured at 5 RPMI (Hyclone) and 2 mM L-glutamine. All growth mediums were supplemented with 10 penicillin. 1x10^5 cells per well in a 6-well plate and harvested after 24hrs. Cells were washed in PBS and fixed in 4 overnight. PFA was removed, and cells were resuspended in 95 samples were then rehydrated in distilled water, stained with DAPI and analyzed by flow cytometry on a LSRII (BD Biosciences) machine. The cell cycle phases ModFit software (Verity Software House) was used to quantify the percentage of cells in each cell cycle phase. Percentages were converted to log2 fractions for each sample. Student's t-test was used for statistical analysis comparing the mock vs miR34a asRNA overespression conditions using five (PC3) or three (Skov3) independant experiments.

Format

A tibble with 12 rows and 6 variables:

'Biological Replicate'

Numeric; Indicates the biological replicate of the sample.

'Cell line'

Character; Indicates the cell line the experiment was performed in.

condition

Factor; indicates the genetic modifications in the cell line.

phase

Factor; The cell cycle phase.

value

Numeric; The percentage of cells in the corresponding phase

. ...

Examples

getData('Figure 3b')

GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.