figure2c: P1 transfections in HCT116 and HEK293t cells.

In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.

Description

All cell lines were cultured at 5 in DMEM high glucose (Hyclone) and HCT116 cells in McCoy<e2><80><99>s 5a (Life Technologies). All growth mediums were supplemented with 10 heat-inactivated FBS and 50 <ce><bc>g/ml of streptomycin and 50 <ce><bc>g/ml of penicillin. Cells were plated at 10,000 cells per well in a 96-well plate with a white bottom and cultured overnight. The following day cells were co-transfected with 10ng of empty, p1, or p2 plasmid and GFP using the standard lipofectamine 2000 (Life Technologies) protocol. The expression of GFP and luminescence was measured 24 h post transfection by using the Dual-Glo Luciferase Assay System (Promega) and detected by the GloMax-Multi+ Detection System (Promega). The expression of luminescence was normalized to GFP.

Format

A tibble with 36 rows and 6 variables:

'Biological Replicate'

Numeric; Indicates the biological replicate of the sample.

'Cell line'

Character; Indicating the cell line the data corresponds to.

gene

Character; indicates the gene that the Ct values correspond to.

alias

Character; Indicating the genes alias.

construct

Character; Indicates the transfected construct.

value

Indicates the activity metric normalized to GFP.

...

Examples

getData('Figure 2c')

GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.