count_cells: Count Cells

In avhgenomics/bioKIT: bioKIT: Common functions for routine molecular biology applications.

Description

count_cells() is a calculator for standard hemocytometry applications.

Usage

count_cells(sampleID = NULL, live, dead = 0, squares_counted = 4,
  sample_aliquot_vol = 0, PBS_vol = 0, Trypan_blue_vol = 0,
  original_sample_vol)

Arguments

sampleID	Provides a label or name for the sample counted.
live	The total number of live cells counted.
dead	The total number of dead cells counted.
squares_counted	How many of the outer corner-squares were counted, Default is 4.
sample_aliquot_vol	The ul of sample taken for hemocytometry.
PBS_vol	The ul of PBS or other solution that the sample aliquot is diluted with. Used to determine the dilution factor.
Trypan_blue_vol	The ul of Trypan Blue, if used. A good starting point is usually 1:1 (sample_aliquot+PBS):Trypan Blue.
original_sample_vol	How many ml is the original set of cells suspended in?

Value

returns a dataframe containing all of the calculations.

Examples

# count_cells(sampleID = "A",live = 300,dead = 10,squares_counted = 4,sample_aliquot_vol = 10,PBS_vol = 40,Trypan_blue_vol = 50,original_sample_vol = 6)

avhgenomics/bioKIT documentation built on May 5, 2019, 12:29 a.m.