#' Function to load breast cancer SummarizedExperiment objects from the
#' Experiment Hub
#'
#' This function returns breast cancer datasets from the hub and a vector of
#' patients from the datasets that are duplicates based on a spearman
#' correlation > 0.98
#'
#' @param rescale apply centering and scaling to the expression sets
#' (default FALSE)
#' @param minNumberGenes an integer specifying to remove expression sets with
#' less genes than this number (default 0)
#' @param minNumberEvents an integer specifying how man survival events must be
#' in the dataset to keep the dataset (default 0)
#' @param minSampleSize an integer specifying the minimum number of patients
#' required in a summarizedExperiment (default 0)
#' @param keepCommonOnly remove entrezIDs not common to all datasets
#' (default FALSE)
#' @param imputeMissing remove patients from datasets with missing expression
#' values
#' @param removeDuplicates remove patients with a Spearman correlation greater
#' than or equal to 0.98 with other patient expression profiles (default TRUE)
#'
#' @return A `list` with 2 elements. The First element named
#' `SummarizedExperiment`s contains the datasets. The second element named
#' duplicates contains a vector with patient IDs for the duplicate patients
#' (those with Spearman correlation greater than or equal to 0.98 with other
#' patient expression profiles).
#'
#' @importFrom Biobase esApply featureNames sampleNames exprs pData
#' experimentData ExpressionSet
#' @importFrom lattice levelplot
#' @importFrom impute impute.knn
#' @importFrom ExperimentHub ExperimentHub
#' @importFrom AnnotationHub query
#' @importFrom stats complete.cases sd quantile
#' @importFrom SummarizedExperiment SummarizedExperiment assays assayNames
#' colData rowData
#' @export
loadBreastDatasets <- function(rescale = FALSE, minNumberGenes = 0,
minNumberEvents = 0, minSampleSize = 0, keepCommonOnly = FALSE,
imputeMissing = FALSE, removeDuplicates = FALSE)
{
duplicates <- NULL
##recursive intersect function
intersectMany <- function(lst){
## Find the intersection of multiple vectors stored as elements of a
## list, through a tail-recursive function.
if (length(lst) == 2){
return(intersect(lst[[1]],lst[[2]]))
}else{
return(intersectMany(c(list(intersect(lst[[1]],lst[[2]])),lst[seq(-1, -2)])))
}
}
## -----------------------------------------------------------------------------
##load the summarizedExperiments
## -----------------------------------------------------------------------------
hub = ExperimentHub::ExperimentHub()
#AnnotationHub::possibleDates(hub)
#query(eh, c("MetaGxOvarian", "SummarizedExperiment"))
breastData = query(hub, c("MetaGxBreast", "SummarizedExperiment"))
#pancreas issues: loading dataset 6, but missing /v1/ for 3 datasets
dataList <- list()
for(i in seq_len(length(breastData)))
{
dataList[[i]] <- breastData[[names(breastData)[i]]]
names(dataList)[i] <- breastData[i]$title
}
names(dataList) <- gsub("_sumexp", "", names(dataList), ignore.case = TRUE)
## -----------------------------------------------------------------------------
##Explicit removal of samples from specified datasets:
## -----------------------------------------------------------------------------
delim <- ":" ##This is the delimiter used to specify dataset:sample,
## same as used in metagx getbrcadata
#load("inst\\extdata\\BenDuplicate.rda")
#source(system.file("extdata", "patientselection.config", package="MetaGxOvarian"))
load(system.file("extdata", "duplicates.rda", package="MetaGxBreast"))
rmix <- duplicates
ii <- 1
while (length(rmix) > ii){
rmix <- rmix [!is.element(names(rmix), rmix[[ii]])]
ii <- ii+1
}
rmix <- unique(unlist(rmix))
rmix <- substr(rmix, unlist(lapply(gregexpr("\\.", rmix),
function(x) x[[1]][1]+1)), nchar(rmix))
message("Clean up the summarizedExperiments")
remInds = c()
for (i in seq_len(length(dataList))){
data <- dataList[[i]]
include <- TRUE
##rescale to z-scores
if(rescale == TRUE){
SummarizedExperiment::assay(data) <-
t(scale(t(SummarizedExperiment::assay(data))))
}
if(removeDuplicates == TRUE){
keepix <- setdiff(colnames(SummarizedExperiment::assay(data)), rmix)
if(length(keepix) != length(colnames(SummarizedExperiment::assay(data))))
{
keepix <- which(!colnames(SummarizedExperiment::assay(data)) %in% rmix)
data <- data[ ,keepix]
}
}
##include study if it has enough samples and events:
phenoData = colData(data)
remData = TRUE
if(nrow(phenoData) - sum(is.na(phenoData$vital_status)) >= minNumberEvents)
remData <- FALSE
if(nrow(phenoData) - sum(is.na(phenoData$recurrence_status)) >= minNumberEvents)
remData <- FALSE
if(nrow(phenoData) >= minSampleSize) remData <- FALSE
if(remData == TRUE){
message(paste("excluding", names(dataList)[i], "(minNumberEvents or minSampleSize)"))
remInds <- c(remInds, i)
include <- FALSE
}
if(nrow(data) < minNumberGenes) {
message(paste("excluding experiment hub dataset",names(dataList)[i],"(minNumberGenes)"))
remInds <- c(remInds, i)
include <- FALSE
}
if(imputeMissing == TRUE){
notNaInds <- which(colSums(is.na(SummarizedExperiment::assay(data))) == 0)
data <- data[, notNaInds]
if(length(notNaInds) == 0){
message(paste("excluding experiment hub dataset",names(dataList)[i],
"as every patient has at least 1 NA expression value (imputmissing = TRUE)"))
remInds <- c(remInds, i)
include <- FALSE
}
}
if(include == TRUE)
message(paste("including experiment hub dataset", names(dataList)[i]))
## featureNames(eset) <- make.names(featureNames(eset))
## should not do this, it is irreversible.
dataList[[i]] <- data
rm(data)
}
if(length(remInds) > 0) dataList[unique(remInds)] = NULL
##optionally take the intersection of genes common to all platforms:
if(keepCommonOnly & length(dataList) > 0){
features.per.dataset <- lapply(dataList, function(x) rowData(x)$EntrezGene.ID)
intersect.genes <- intersectMany(features.per.dataset)
dataList <- lapply(dataList, function(data){
data <- data[which(rowData(data)$EntrezGene.ID %in% intersect.genes), ]
return(data)
})
}
if(length(dataList) == 0)
warning("input values resulted in no datasets being returned")
retList = list(dataList, duplicates)
names(retList) = c("summarizedExperiments", "duplicates")
return(retList)
}
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