make_seurat_annot: Creation of Seurat object

In dkzhang777/SlideCNA: Calls Copy Number Alterations from Slide-Seq Data

Creation of Seurat object

Description

This function takes in raw counts (and potentially meta data) to make a Seurat object and process it

Usage

make_seurat_annot(
  cb,
  md = NULL,
  seed_FindClusters = 0,
  seed_RunTSNE = 1,
  seed_RunUMAP = 42
)

Arguments

cb	sparse counts matrix (genes x cells/beads)
md	data.frame of meta data for cells/beads if specific annotations known
seed_FindClusters	seed number for FindCLusters
seed_RunTSNE	seed number for RunTSNE
seed_RunUMAP	seed number for RunUMAP

Value

A Seurat object with specific Seurat features run

dkzhang777/SlideCNA documentation built on Jan. 25, 2025, 5:53 p.m.