agilent.miRNA.normalise.GX10: Normalise Agilent miRNA data Normalise an Agilent miRNA data...
In drmjc/microarrays: A collection of microarray and genomic helper code.
Description
Usage
Arguments
Value
Author(s)
Description
Normalise Agilent miRNA data Normalise an Agilent miRNA
data set using the default method from Agilent GeneSpring
GX 10 which is percentile shift normalisation. From the
GX 10 manual:
18.2.1 Percentile Shift Normalization
Percentile shift normalization is a global normalization,
where the location of all the spot intensities in an
array are adjusted. This normalization takes each column
in an experiment independently, and computes the
percentile of the expression values for this array,
across all spots (where n has a range from 0-100 and n=50
is the median). It subtracts this value from the
expression value of each entity.
Usage
1
agilent.miRNA.normalise.GX10(data, pctile = 0.75)
Arguments
data
a list of imported, thresholded,
log-base-2 Agilent data. see
agilent.miRNA.threshold.GX10
pctile
the percentile to normalize to.
default=0.75, ie the 75th percentile. Must be in [0,1]
Value
a list of normalised miRNA data
Author(s)
Mark Cowley, 2009-07-22
drmjc/microarrays documentation built on May 15, 2019, 2:26 p.m.
R Package Documentation
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Description Usage Arguments Value Author(s)
Description
Normalise Agilent miRNA data Normalise an Agilent miRNA
data set using the default method from Agilent GeneSpring
GX 10 which is percentile shift normalisation. From the
GX 10 manual:
18.2.1 Percentile Shift Normalization
Percentile shift normalization is a global normalization,
where the location of all the spot intensities in an
array are adjusted. This normalization takes each column
in an experiment independently, and computes the
percentile of the expression values for this array,
across all spots (where n has a range from 0-100 and n=50
is the median). It subtracts this value from the
expression value of each entity.
Usage
1 | agilent.miRNA.normalise.GX10(data, pctile = 0.75)
|
Arguments
data |
a |
pctile |
the percentile to normalize to. default=0.75, ie the 75th percentile. Must be in [0,1] |
Value
a list of normalised miRNA data
Author(s)
Mark Cowley, 2009-07-22
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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