agilent.miRNA.preprocess: Preprocess Agilent miRNA data. From a table of imported...

Description Usage Arguments Value Author(s)

Description

Preprocess Agilent miRNA data. From a table of imported Agilent data, add an offset, truncate, log-transform, normalize and plot a set of Agilent microarray files. This combines transform_Agilent_log, agilent.miRNA.normalise.GX10 or normalizeBetweenArrays, and agilent.miRNA.filter.GX10

Usage

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  agilent.miRNA.preprocess(data, offset = 8,
    min.thresh = 1, percentile = 0.75, min.Pcount = 1,
    species = "mmu", plot = FALSE, do.par = TRUE,
    norm.method = c("percentile", "none", "scale", "quantile"))

Arguments

data

a list of imported Agilent data. see import.agilent

offset

add an offset. see transform_Agilent_log

min.thresh

if any values are < this value, then truncate them to this value. see transform_Agilent_log

percentile

The percentile to perform percentile-shift normalization. only used if norm.method=="percentile", see agilent.miRNA.normalise.GX10

min.Pcount

integer exclude genes detected in fewer than min.Pcount samples. see agilent.miRNA.filter.GX10

species

the 3 letter code for which species of miRNA's to include. see agilent.miRNA.filter.GX10

plot

logical: add boxplots of data during the transformation process

do.par

logical: configure the plotting device settings?

norm.method

The normalisation method to use. one of “percentile”, “none”, “scale”, “quantile”. If “percentile”, then make sure you set the percentile parameter appropriately. If “scale” or “quantile”, then the normalizeBetweenArrays from limma is used. If “none” then no normalisation is performed.

Value

a list of log-transformed, filtered, normalised Agilent microRNA data.

Author(s)

Mark Cowley, 2011-08-02


drmjc/microarrays documentation built on May 15, 2019, 2:26 p.m.