agilent.miRNA.preprocess.GX10: Preprocess Agilent miRNA data Preprocess raw Agilent data,...

Description Usage Arguments Value Author(s)

Description

Preprocess Agilent miRNA data Preprocess raw Agilent data, then threshold it to a minimum value, log transform, normalise each array such that the 75th percentile is 0.0, throw away miRs from other species, and keep those that are detected at least min.Pcount times. Optionally makes a 2x2 portrait layout plot @ each step. This is the default procedure by GeneSpring GX 10 (not sure about newer versions)

Usage

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  agilent.miRNA.preprocess.GX10(data, min.thresh = 1,
    percentile = 0.75, min.Pcount = 1, species = "mmu",
    plot = FALSE, do.par = TRUE)

Arguments

data

a list of imported Agilent data. see import.agilent

min.thresh

if any values are < this value, then truncate them to this value. see agilent.miRNA.threshold.GX10

percentile

The percentile to perform percentile-shift normalization. See agilent.miRNA.normalise.GX10

min.Pcount

integer exclude genes detected in fewer than min.Pcount samples. see agilent.miRNA.filter.GX10

species

the 3 letter code for which species of miRNA's to include. see agilent.miRNA.filter.GX10

plot

logical: add boxplots of data during the transformation process

do.par

logical: configure the plotting device settings?

Value

a set of thresholded, log-transformed, filtered, normalized Agilent miRNA data

Author(s)

Mark Cowley, 2009-07-22


drmjc/microarrays documentation built on May 15, 2019, 2:26 p.m.