Description Usage Arguments Value
Function to produce files necessary to generate 16S sequencing runs that utilizes the Beckmann robot for dilutions and creates sequencing mapping files in QIIME-ready format, from multiple experiment data sets.
1 2 | sequencing_prep_16S(experiment_data_list, n_barcode_plates,
barcode_map_file = NULL, output_directory = getwd(), filter = T)
|
experiment_data_list |
an input list that contains the data from the samples you want to sequence (ideally, the output from DataAnalysis, or combinations of them) |
n_barcode_plates |
the number of barcode plates available (i.e. if you have 96 unique sequencing barcodes, this would be 1) |
barcode_map_file |
a string of the path to a file containing the barcode sequences, linker primer sequences, and barcode plate and well locations (see example data) |
output_directory |
OPTIONAL: a path to the directory for your desired output (Default = working directory) |
filter |
OPTIONAL: a boolean that indicates whether or not to filter out samples that have not passed the QC check from the DataAnalysis function (Default = TRUE) |
A list containing the data for as many sequencing runs necessary to sequence all of your samples. For each sequencing run, there will be a list of plates, corresponding to the data frames that contain the information necessary to use the Beckmann robot to dilute samples. There will also be a mapping file, and a list of samples included in that sequencing run.
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