tests/testthat/test_runAbsoluteCN.R

In lima1/PureCN: Copy number calling and SNV classification using targeted short read sequencing

context("runAbsoluteCN")

normal.coverage.file <- system.file("extdata", "example_normal.txt.gz",
    package = "PureCN")
normal2.coverage.file <- system.file("extdata", "example_normal2.txt.gz",
    package = "PureCN")
normal.coverage.files <- c(normal.coverage.file, normal2.coverage.file)
tumor.coverage.file <- system.file("extdata", "example_tumor.txt.gz",
    package = "PureCN")
vcf.file <- system.file("extdata", "example.vcf.gz", package = "PureCN")
interval.file <- system.file("extdata", "example_intervals.txt",
    package = "PureCN")
seg.file <- system.file("extdata", "example_seg.txt", package = "PureCN")
cosmic.vcf.file <- system.file("extdata", "example_cosmic.vcf.gz",
    package = "PureCN")
data(purecn.example.output)

test_that("VCF is not necessary to produce output", {
    set.seed(123)
    normalDB <- createNormalDatabase(normal.coverage.files)
    expect_true(!is.null(normalDB$sd))
    ret <- runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor.coverage.file,
        candidates = purecn.example.output$candidates,
        normalDB = normalDB,
        genome = "hg19",
        test.purity = seq(0.4, 0.7, by = 0.05), min.ploidy = 1.5,
        max.ploidy = 2.4, max.candidate.solutions = 1,
        BPPARAM = BiocParallel::bpparam())
    
    expect_true(!is.null(ret$version))
    tmpFile <- tempfile(fileext = ".rds")
    saveRDS(ret, tmpFile)
    createCurationFile(tmpFile)
    readCurationFile(tmpFile)
    file.remove(tmpFile)
    chrom <- ret$results[[1]]$seg$chrom
    expect_equal(chrom[!duplicated(chrom)], 1:22)
    expect_error(callLOH(ret), "runAbsoluteCN was run without a VCF file")
    expect_error(callMutationBurden(ret),
        "runAbsoluteCN was run without a VCF file")

    expect_error(runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor.coverage.file,
        normalDB = normalDB,
        genome = "hg19",
        fun.segmentation = segmentationPSCBS),
        "segmentationPSCBS requires VCF")

    expect_output(ret <- runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor.coverage.file,
        candidates = purecn.example.output$candidates,
        normalDB = normalDB,
        vcf.file = vcf.file,
        genome = "hg19",
        test.purity = seq(0.4, 0.7, by = 0.05), min.ploidy = 1.5,
        max.ploidy = 2.4, max.candidate.solutions = 1,
        min.variants = 1000),
        "Insufficient number of variants")
    expect_true(is.null(ret$results[[1]]$SNV.posterior))
})

test_that("Exceptions happen with incorrect input data", {
    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        genome = "hg19"),
        "Need a normal coverage file if log.ratio and seg.file")
    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        min.ploidy = 0, genome = "hg19"),
        "min.ploidy or max.ploidy not within expected range")
    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        max.ploidy = 0, genome = "hg19"),
    "min.ploidy or max.ploidy not within expected range")
    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        normal.coverage.file = normal.coverage.file, max.ploidy = "a",
        genome = "hg19"), "max.ploidy")
    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        normal.coverage.file = normal.coverage.file, min.ploidy = "a",
        genome = "hg19"), "min.ploidy")
    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        normal.coverage.file = normal.coverage.file, test.num.copy = -1:7,
        genome = "hg19"), "test.num.copy")
    expect_error(expect_warning(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        normal.coverage.file = normal.coverage.file, test.num.copy = 0:10,
        genome = "hg19", max.non.clonal = 1.1), "test.num.copy outside"))
    expect_error(expect_warning(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        normal.coverage.file = normal.coverage.file, test.num.copy = 2:7,
        genome = "hg19", max.non.clonal = 1.1), "test.num.copy outside"))

    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        normal.coverage.file = normal.coverage.file, test.purity = "a",
        genome = "hg19"), "test.purity")
    expect_error(runAbsoluteCN(tumor.coverage.file, tumor.coverage.file,
        genome = "hg19"), "Tumor and normal are identical")
    expect_error(runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        log.ratio = head(purecn.example.output$input$log.ratio$log.ratio),
        genome = "hg19"),
        "Length of log.ratio different from tumor coverage")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        prior.purity = 1, genome = "hg19"),
        "prior.purity must have the same")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        min.gof = 70, genome = "hg19"), "min.gof")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        prior.purity = c(0.6, 1.1), test.purity = c(0.2, 0.6), 
        genome = "hg19"),
        "prior.purity not within expected range")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        prior.purity = c(0.6, 0.9), test.purity = c(0.2, 0.6),
        genome = "hg19"),
        "prior.purity must add to 1. Sum is 1.5")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", max.homozygous.loss = 1.1),
        "max.homozygous.loss not within expected range")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", prior.K = -1.1),
        "prior.K not within expected range")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", prior.contamination = c(0.1, -1.1)),
        "prior.contamination not within expected range")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", iterations = 1),
        "Iterations not in the expected range from")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", iterations = 3000),
        "Iterations not in the expected range from")
    expect_error(runAbsoluteCN(normal.coverage.file = normal.coverage.file),
        "Missing tumor")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", model.homozygous = NULL), "model.homozygous")
    normalCov <- readCoverageFile(normal.coverage.file)
    expect_error(runAbsoluteCN(normalCov[sample(length(normalCov)),
        ], tumor.coverage.file, genome = "hg19"),
        "Interval files in normal and tumor different")
    tumorCov <- readCoverageFile(tumor.coverage.file)
    tumorCov$average.coverage <- 0
    tumorCov$coverage <- 0
    expect_error(runAbsoluteCN(normal.coverage.file = normal.coverage.file,
          tumor.coverage.file = tumorCov, genome = "hg19"),
          "intervals")
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", vcf.file = vcf.file,
        args.filterVcf = list(snp.blacklist = normal.coverage.file)),
        "Could not import snp.blacklist")
})


test_that("Example data with VCF produces expected output", {
    ret <- runAbsoluteCN(normal.coverage.file = normal.coverage.file, 
        tumor.coverage.file = tumor.coverage.file, vcf.file = vcf.file,
        genome = "hg19", test.purity = seq(0.3, 0.7, by = 0.05), plot.cnv = FALSE,
        args.filterIntervals = list(min.total.count = 0),
        max.candidate.solutions = 1, min.ploidy = 1.5, max.ploidy = 2.1,
        vcf.field.prefix = "PureCN."
    )
    expect_true(!is.null(ret$version))
    expect_equal(ret$results[[1]]$fraction.balanced, 0.2, tolerance = 0.02)
    s <- predictSomatic(ret)
    expect_equal(s$AR / s$MAPPING.BIAS, s$AR.ADJUSTED)
    expect_equal(0.65, ret$results[[1]]$purity)
    expect_equal(0.92, ret$results[[1]]$GoF, tolerance = 0.02)
    expect_equal(as.numeric(colnames(ret$candidates$all)), c(seq(0.3,
        0.34, by = 1 / 50), seq(0.35, 0.7, by = 1 / 30)))
    plotAbs(ret, type = "overview")
    expect_equal(info(ret$input$vcf)$PureCN.OnTarget, rep(1, length(ret$input$vcf)))
    expect_equal(0.3195055, ret$input$mapd$all, tolerance = 0.025)
})

test_that("Mapping bias function works", {
    vcf <- readVcf(vcf.file, "hg19", param = ScanVcfParam(samples = "LIB-02240e4"))
    # make sure that NA's in DB are replaced with FALSE
    info(vcf)$DB[1:5] <- NA
    geno(vcf)$DP[6:8, 1] <- NA
    myMappingBiasTestFun <- function(vcf, ...) {
        tmp <- rep(1, nrow(vcf))
        tmp2 <- rep(0, nrow(vcf))
        idx <- as.logical(seqnames(vcf) == "chr9" & start(vcf) ==
            35811642)
        tmp[idx] <- 0.9
        tmp2[idx] <- 2
        return(PureCN:::.annotateMappingBiasVcf(vcf,
                    data.frame(bias = tmp, pon.count = tmp2, mu = NA, rho = NA)))
    }
    ret <- runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor.coverage.file, args.filterVcf = list(remove.off.target.snvs = FALSE),
        vcf.file = vcf, genome = "hg19", test.purity = seq(0.3,
            0.7, by = 0.05), min.ploidy = 1.5, max.ploidy = 2.1,
        max.candidate.solutions = 1, fun.setMappingBiasVcf = myMappingBiasTestFun)
    expect_equal(0.65, ret$results[[1]]$purity)
    expect_equal(as.numeric(colnames(ret$candidates$all)), c(seq(0.3,
        0.34, by = 1 / 50), seq(0.35, 0.7, by = 1 / 30)))
    s <- predictSomatic(ret)
    expect_equal(s$AR/s$MAPPING.BIAS, s$AR.ADJUSTED)
    expect_equal(s$MAPPING.BIAS[s$chr == "chr9" & s$start ==
        35811642], 0.9)
    expect_equal(s$pon.count[s$chr == "chr9" & s$start == 35811642],
        2)
})

test_that("Missing Gene column in interval.file is handled correctly", {
    gc_data <- read.delim(interval.file, as.is = TRUE)
    gc_data$Gene <- NULL
    output.file <- tempfile(".txt")
    write.table(gc_data, file = output.file, row.names = FALSE, sep = "\t",
        quote = FALSE)
    ret <- runAbsoluteCN(normal.coverage.file = normal.coverage.file,
                         fun.segmentation = segmentationPSCBS,
        interval.file = output.file, model.homozygous = TRUE, tumor.coverage.file = tumor.coverage.file,
        candidates = purecn.example.output$candidates,
        min.ploidy = 2.2, max.ploidy = 4, vcf.file = vcf.file,
        genome = "hg19", test.purity = seq(0.3, 0.7, by = 0.05),
        max.candidate.solutions = 1)
    expect_true(ret$results[[1]]$ploidy <= 4)
    expect_true(ret$results[[1]]$ploidy >= 2)
    expect_true(is.na(ret$results[[1]]$gene.calls))
    expect_error(callAlterations(ret), "requires gene-level calls")
    rvcf <- predictSomatic(ret, return.vcf = TRUE)
    expect_equal(length(ret$input$vcf), 
                 length(rvcf))
     expect_equal(".", info(rvcf)$GS[1])

    tumor <- readCoverageFile(tumor.coverage.file)
    normal <- readCoverageFile(normal.coverage.file)
    tumor$gc_bias <- gc_data$gc_bias
    tumor$normal.average.coverage <- normal$average.coverage
    filtered <- tumor[!overlapsAny(tumor, ret$input$log.ratio)]
    expect_equal(sum(!(filtered$average.coverage < 15 | filtered$normal.average.coverage <
        15 | filtered$targeted.base < 5 | filtered$gc_bias <
        0.25 | filtered$gc_bias > 0.8)), 0)
    file.remove(output.file)
})

test_that("Different chromosome naming styles throw exceptions", {
    vcf <- readVcf(vcf.file, "hg19")
    seqlevelsStyle(vcf) <- "Ensembl"
    normCov <- readCoverageFile(normal.coverage.file)
    tumorCov <- readCoverageFile(tumor.coverage.file)
    seqlevelsStyle(normCov) <- "Ensembl"
    seqlevelsStyle(tumorCov) <- "Ensembl"
    expect_error(runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        genome = "hg19", vcf.file = vcf),
        "Different chromosome names in coverage and VCF")
    expect_error(runAbsoluteCN(normal.coverage.file = normCov,
        tumor.coverage.file = tumorCov, interval.file = interval.file,
        vcf.file = vcf, genome = "hg19", test.purity = seq(0.3,
            0.7, by = 0.05), max.candidate.solutions = 1),
        "tumor.coverage.file and interval.file do not")

    output.file1 <- tempfile(fileext = ".txt")
    output.file2 <- tempfile(fileext = ".txt")
    gc1 <- read.delim(interval.file, as.is = TRUE)
    gc1[, 1] <- as.character(tumorCov)
    write.table(gc1, file = output.file1, row.names = FALSE, sep = "\t",
        quote = FALSE)
    gc2 <- gc1
    gc2$Gene[2:4] <- paste("SCNN1D,XXXL")
    gc2$Gene[1002:1004] <- paste("PXDN,XXXL")
    write.table(gc2, file = output.file2, row.names = FALSE, sep = "\t",
        quote = FALSE)
    set.seed(123)
    ret <- runAbsoluteCN(normal.coverage.file = normCov, tumor.coverage.file = tumorCov,
        interval.file = output.file1, plot.cnv = FALSE, min.ploidy = 1.5,
        max.ploidy = 2.1, vcf.file = vcf, genome = "hg19", test.purity = seq(0.4,
            0.7, by = 0.05), max.candidate.solutions = 1)
    set.seed(123)
    ret2 <- runAbsoluteCN(normal.coverage.file = normCov, tumor.coverage.file = tumorCov,
        interval.file = output.file2, plot.cnv = FALSE, min.ploidy = 1.5,
        max.ploidy = 2.1, vcf.file = vcf, genome = "hg19", test.purity = seq(0.4,
            0.7, by = 0.05), max.candidate.solutions = 1)
    gpnmb <- callAlterations(ret)["GPNMB", ]
    expect_equal(as.character(gpnmb$chr), "7")
    expect_true(gpnmb$start > 2.3e+07)
    expect_true(gpnmb$end < 23400000)
    expect_true(gpnmb$C >= 6)
    expect_equal(gpnmb$type, "AMPLIFICATION")
    caAret <- callAlterations(ret, all.genes = TRUE)
    caAret2 <- callAlterations(ret2, all.genes = TRUE)
    expect_equal(nrow(caAret2), nrow(caAret) + 2)
    expect_equal(caAret2[rownames(caAret), "C"], caAret$C)
    expect_equal(caAret2[rownames(caAret), "start"], caAret$start)
    expect_equal(caAret2["XXXL__1", "number.targets"], 3)
    expect_equal(caAret2["XXXL__1", "start"], 1216606)
    expect_equal(caAret2["XXXL__1", "end"], 1217696)
    expect_equal(caAret2["XXXL__1", "C"], caAret["SCNN1D", "C"])
    expect_equal(caAret2["XXXL__1", "seg.mean"], caAret["SCNN1D",
        "seg.mean"])
    expect_equal(caAret2["XXXL__2", "number.targets"], 3)
    expect_equal(caAret2["XXXL__2", "start"], 1695700)
    expect_equal(caAret2["XXXL__2", "end"], 1748228)
    expect_equal(caAret2["XXXL__2", "C"], caAret["PXDN", "C"])
    expect_equal(caAret2["XXXL__2", "seg.mean"], caAret["PXDN",
        "seg.mean"])
    plotAbs(ret, 1, type = "all")
    expect_error(plotAbs(ret, 1, type = "BAF", chr = "chr1"))
    plotAbs(ret, 1, type = "BAF", chr = "1")
    x <- ret$results[[1]]$gene.calls
    seg <- ret$results[[1]]$seg
    expect_equal(x$breakpoints == 0, x$.min.segid == x$.max.segid)
    expect_equal(seg$seg.mean[x$seg.id], x$seg.mean)
    expect_equal(seg$C[x$seg.id], x$C)

    file.remove(c(output.file1, output.file2))
})

test_that("Run with example seg.file works", {
    ret <- runAbsoluteCN(tumor.coverage.file = tumor.coverage.file,
        seg.file = seg.file, vcf.file = vcf.file, max.candidate.solutions = 1,
        genome = "hg19", min.ploidy = 1.5, max.ploidy = 2.1,
        test.purity = seq(0.4, 0.7, by = 0.05), sampleid = "Sample2")
    expect_equal(0.65, ret$results[[1]]$purity)

    # check for https://github.com/lima1/PureCN/issues/19
    vcf <- purecn.example.output$input$vcf
    vcf.id <- match("chr2231775021xxx", names(vcf))
    end(vcf[vcf.id]) <- 236403333

    ret <- runAbsoluteCN(seg.file = seg.file, interval.file = interval.file,
        vcf.file = vcf, max.candidate.solutions = 1, genome = "hg19",
        test.purity = seq(0.4, 0.7, by = 0.05), min.ploidy = 1.5,
        max.ploidy = 2.1, verbose = FALSE)
    expect_equal(0.65, ret$results[[1]]$purity)
    tmp <- predictSomatic(ret)[ret$results[[1]]$SNV.posterior$vcf.ids == vcf.id, ]
    expect_equal(2, nrow(tmp))
    expect_equal(2, sum(tmp$FLAGGED))
    expect_equal(rep("chr2231775021xxx", 2), as.character(tmp$ID))
    tmp <- read.delim(seg.file, as.is = TRUE)
    colnames(tmp)[1:4] <- c("Name", "Chromosome", "Start", "End")
    output.file <- tempfile(fileext = ".seg")
    write.table(tmp, file = output.file, quote = FALSE, row.names = FALSE,
        sep = "\t")
    expect_error(runAbsoluteCN(seg.file = output.file, interval.file = interval.file,
        vcf.file = vcf.file, max.candidate.solutions = 1, genome = "hg19",
        min.ploidy = 1.4, max.ploidy = 2.4, test.purity = seq(0.4,
            0.7, by = 0.05), verbose = FALSE))
    file.remove(output.file)
    tmp <- read.delim(seg.file, as.is = TRUE)
    tmp2 <- tmp
    tmp2[, 1] <- "Sample2"
    tmp <- rbind(tmp, tmp2)
    output.file <- tempfile(fileext = ".seg")
    write.table(tmp, file = output.file, quote = FALSE, row.names = FALSE,
        sep = "\t")
    expect_error(runAbsoluteCN(seg.file = output.file, interval.file = interval.file,
        vcf.file = vcf.file, max.candidate.solutions = 1, genome = "hg19",
        min.ploidy = 1.4, max.ploidy = 2.4, test.purity = seq(0.4,
            0.7, by = 0.05), verbose = FALSE),
        "seg.file contains multiple samples and sampleid missing")
    expect_error(runAbsoluteCN(seg.file = output.file, interval.file = interval.file,
        sampleid = "Sample3", vcf.file = vcf.file, max.candidate.solutions = 1,
        genome = "hg19", test.purity = seq(0.3, 0.7, by = 0.05),
        verbose = FALSE),
        "contains multiple samples and sampleid does not match")
    ret <- runAbsoluteCN(seg.file = output.file, interval.file = interval.file,
        sampleid = "Sample1", vcf.file = vcf.file, max.candidate.solutions = 1,
        fun.segmentation = segmentationHclust,
        genome = "hg19", test.purity = seq(0.4, 0.7, by = 0.05),
        verbose = FALSE, min.ploidy = 1.5, max.ploidy = 2.1)
    file.remove(output.file)

    testSeg <- function(seg, ...) return(seg)
    res <- runAbsoluteCN(normal.coverage.file, tumor.coverage.file,
        seg.file = seg.file, fun.segmentation = testSeg, min.ploidy = 1.5,
        max.ploidy = 2.1, test.purity = seq(0.4, 0.7, by = 0.05),
        max.candidate.solutions = 1, genome = "hg19", plot.cnv = FALSE)
    seg <- read.delim(seg.file)
    expect_equal(nrow(res$results[[1]]$seg), nrow(seg))
    expect_equal(res$results[[1]]$seg$seg.mean, seg$seg.mean, tol = 0.005)
})

test_that("Run with provided log-ratios works", {
    log.ratio <- calculateLogRatio(readCoverageFile(normal.coverage.file),
        readCoverageFile(tumor.coverage.file))
    ret <- runAbsoluteCN(log.ratio = log.ratio, interval.file = interval.file,
        vcf.file = vcf.file, max.candidate.solutions = 1, genome = "hg19",
        min.ploidy = 1.5, max.ploidy = 2.1, plot.cnv = FALSE,
        test.purity = seq(0.4, 0.7, by = 0.05))
    expect_equal(0.65, ret$results[[1]]$purity)
    ret <- runAbsoluteCN(log.ratio = log.ratio, seg.file = seg.file,
        interval.file = interval.file, vcf.file = vcf.file, max.candidate.solutions = 1,
        min.ploidy = 1.5, max.ploidy = 2.1,
        genome = "hg19", test.purity = seq(0.5, 0.7, by = 0.05))
    expect_equal(0.65, ret$results[[1]]$purity)
})

test_that("Betabin model runs with example data", {
    vcf <- readVcf(vcf.file, "hg19")
    gt1 <- round(geno(vcf)$DP[, 1] * as.numeric(geno(vcf)$FA[,
        1]))
    ad1 <- lapply(seq_along(gt1), function(i) as.numeric(c(geno(vcf)$DP[i,
        1] - gt1[i], gt1[i])))
    names(ad1) <- names(geno(vcf)$DP[, 1])
    geno(vcf)$AD[, 1] <- ad1
    geno(vcf)$FA <- NULL
    geno(vcf)$DP <- NULL
    geno(vcf)$BQ[, 2] <- geno(vcf)$BQ[, 1]
    ret <- runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor.coverage.file, sampleid = "LIB-02252e4",
        vcf.file = vcf, max.candidate.solutions = 1, genome = "hg19",
        min.ploidy = 1.5, max.ploidy = 2.1, plot.cnv = FALSE,
        cosmic.vcf.file = cosmic.vcf.file, model = "betabin",
        test.purity = seq(0.4, 0.7, by = 0.05))
    expect_equal(0.65, ret$results[[1]]$purity, tol = 0.1)
})

test_that("normalDB objects are used correctly", {
    expect_error(runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor.coverage.file, genome = "hg19",
        normalDB = vcf.file),
        "normalDB not a valid normalDB object")

    normalDB <- createNormalDatabase(normal.coverage.files)
    tmp <- normalDB
    tmp$normal.coverage.files <- NULL
    expect_error(runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor.coverage.file, genome = "hg19",
        normalDB = tmp), "normalDB appears to be empty")

    tumor <- readCoverageFile(tumor.coverage.file)
    tumor[1]$on.target <- FALSE

    ret <- runAbsoluteCN(normal.coverage.file = normal.coverage.file,
        tumor.coverage.file = tumor, genome = "hg19",
        vcf.file = vcf.file, sampleid = "Sample1", interval.file = interval.file,
        normalDB = normalDB, args.filterIntervals = list(filter.lowhigh.gc = 0),
        plot.cnv = FALSE, min.ploidy = 1.5, max.ploidy = 2.1, test.purity = seq(0.4,
            0.7, by = 0.05), max.candidate.solutions = 1)
})

test_that("Issue 192 is fixed", {
    expect_error(
    runAbsoluteCN(tumor = system.file("extdata", "issue192_tumor.cnr",
                                      package = "PureCN"),
                  seg.file = system.file("extdata", "issue192_tumor.seg",
                                         package = "PureCN"),
                  sampleid = "tumor",
                  genome = "hg19",
                  fun.segmentation = segmentationHclust),
    "Seqlevels missing in provided segmentation")
})