Description Usage Arguments Value Examples
This function extracts CellTags from the raw fastq/bam sequencing file. If it is a fastq file, provides counts of each CellTag and sorts them in desending order. If it is a bam file, returns the barcode, umi, celltag information.
1 2 | CellTagExtraction(fastq.bam.input, celltag.version,
extraction.output.filename, save.fullTag = TRUE, save.onlyTag = TRUE)
|
fastq.bam.input |
The input fastq/bam data directory |
extraction.output.filename |
The output file directory, i.e. where would you like to store the 8nt tags with their counts? |
save.fullTag |
Would you like to save full sequences with the short sequences provided without counts? Defaults to TRUE |
save.onlyTag |
Would you like to save only the 8nt CellTags without counts? Defaults to TRUE |
short.nt.before.tag |
A short sequence before the 8nt tag to help more specific identification |
short.nt.after.tag |
A short sequence after the 8nt tag to help more specific identification |
A list contains count table of CellTags. If requested to save fullTag counts, i.e. save.fullTag.counts = TRUE, return a list of both 8nt tags and full sequences count. Otherwise, a list of 8nt tags counts.
1 | CellTagExtraction("data.fastq", "v1", "~/Desktop/whitelist.txt")
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