Description Usage Arguments Details Value
Calcuates either percent input or fold enrichment for chip qpcr data
1 2 | do_chip_qpcr(sampleObj, inputName = NULL, mockName = NULL,
inputPercent = NULL, method, bioReps = FALSE)
|
sampleObj |
A qPCRBatch object generated from the |
inputName |
A character vector of the common name given to all input samples |
mockName |
A character vector of the common name given to all mock (e.g. IgG control) samples |
inputPercent |
The percent of starting chromatin used as input. Should be an integer in percent (e.g. 2% input is 2). |
method |
Indicates which calculation should be performed. Must be one of ("i", "input") for input, or ("fe", "fold enrichment") for fold enrichment. |
bioReps |
Boolean indicating whether or not there are biological replicates. |
This function will handle both single replicate or n > 1 replicate experiments. Providing a 'mockName' for percent input calculations will remove the mock samples from the calculations. Likewise, providing an 'inputName' for fold enrichment calculations will remove the input samples from the calculations.
A data.frame with dCT values, percent input/fold enrichment, and max/min percent input/fold enrichment per sample.
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