get_dCT_singleRep: get_dCT_singlerep
In sccallahan/WARPqpcr: Web App and R Package for qPCR

Description Usage Arguments Details Value

Calculates the dCT values for all samples in situations where there is only a single biological replicate per condition.

1	get_dCT_singleRep(sampleObj, hkg)

`sampleObj`	A qPCRBatch object generated from the `readSampleSheet` function.
`hkg`	A character vector containing the housekeeping gene to use for dCT calculations.

This function is primarily intended for pilot/preliminary data in which biological replicates have not yet been produced or cases in which multiple vectors are being evaluated for efficiency (e.g. shRNA knockdowns, overexpression constructs, etc.)

The error for these calculations is propagated from the technical replicate standard deviation.

A data.frame containing dCT values for each condition.

sccallahan/WARPqpcr documentation built on March 1, 2020, 1:23 a.m.