readSampleSheet: readSampleSheet

In sccallahan/WARPqpcr: Web App and R Package for qPCR

Description

Reads in a tab-delimited file of qPCR data using read.qPCR. Requires 5 columns: Well, Plate, Sample, Detector, Cq.

Usage

readSampleSheet(file)

Arguments

file	Tab-delimted file containing qPCR data

Details

Biological replicates MUST be given unique identifiers, else they will be treated as technical replicates. For example, if an experiment has 2 biological replicates for a wildtype sample, they should be entered as "WT_1" and "WT_2". Entering them both as "WT" will result in them being treated as technical replicates of the same sample. Multiple technial replicates of biological replicates should entered with the same names (e.g. "WT_1", "WT_1", "WT_2", "WT_2" would be 2 technical replicates of biological replicate 1 and 2 technical replicates of biological replicate 2).

Value

A qPCRBatch object.

sccallahan/WARPqpcr documentation built on March 1, 2020, 1:23 a.m.