calcStab: calcStab

In sccallahan/WARPqpcr: Web App and R Package for qPCR

Description

Finds the most stable housekeeping gene across conditions using the DeltaCt approach from Silver et al. (2006).

Usage

calcStab(avgCTobj, hkgs)

Arguments

avgCTobj	A data.frame generated from the getAvgCT function.
hkgs	A character vector containing the potential housekeeping genes.

Value

A data.frame containing the tested housekeeping genes and their scores, sorted from lowest to highest (i.e. most stable to least stable).

References

Silver et al. 2006. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol

Examples

## Not run: 
geneRank <- calcStab(avgCTobj, c("ACTB", "GAPDH"))

## End(Not run)

sccallahan/WARPqpcr documentation built on March 1, 2020, 1:23 a.m.