get_ddCT_singleRep: get_ddCT_singleRep

In sccallahan/WARPqpcr: Web App and R Package for qPCR

Description

Calculates the ddCT values for all samples in situations where there is only a single biological replicate per condition.

Usage

get_ddCT_singleRep(sampleObj, hkg, control, rel.exp = FALSE)

Arguments

sampleObj	A qPCRBatch object generated from the readSampleSheet function.
hkg	A character vector containing the housekeeping gene to use for ddCT calculations.
control	A character vector containing the name of the control condition.
rel.exp	Boolean value indicating whether or not relative expression values should be calculated. TRUE will compute both log2 fold-change and expression relative to the control sample, FALSE will only compute log2 fold-change values.

Details

This function is primarily intended for pilot/preliminary data in which biological replicates have not yet been produced or cases in which multiple vectors are being evaluated for efficiency (e.g. shRNA knockdowns, overexpression constructs, etc.)

Value

A data.frame containing ddCT values in log2 fold-change or relative expression format.

Examples

## Not run: 
sampleObj <- readSampleSheet(file)
ddCTObj <- get_ddCT_singleRep(sampleObj, "GAPDH", "untreated",
rel.exp = FALSE)

## End(Not run)

sccallahan/WARPqpcr documentation built on March 1, 2020, 1:23 a.m.