get_chip_dCT: get_chip_dCT

Description Usage Arguments Details Value

View source: R/get_chip_dCT.R

Description

Calculates dCT values for chip qpcr data and organizes it into a format compatible with signifTest.

Usage

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get_chip_dCT(sampleObj, inputName = NULL, mockName = NULL,
  inputPercent = NULL, method)

Arguments

sampleObj

A qPCRBatch object generated from the readSampleSheet function.

inputName

A character vector of the common name given to all input samples

mockName

A character vector of the common name given to all mock (e.g. IgG control) samples

inputPercent

The percent of starting chromatin used as input. Should be an integer in percent (e.g. 2% input is 2).

method

Indicates which calculation should be performed. Must be one of ("i", "input") for input, or ("fe", "fold enrichment") for fold enrichment.

Details

This function only works with biological replicate data. Providing a 'mockName' for percent input calculations will remove the mock samples from the calculations. Likewise, providing an 'inputName' for fold enrichment calculations will remove the input samples from the calculations.

Value

A data.frame


sccallahan/WARPqpcr documentation built on March 1, 2020, 1:23 a.m.