#' Count the degrees of freedom for each group
#'
#' Counts the number of observations within each group for each peptide and
#' divides by the number of replicates -- defaults to one replicate per sample.
#' @param dat a data frame containing samples and observations to be counted
#' @param col a numeric vector containing the column numbers of the group to be
#' counted
#' @param rep number of replicates for each sample defaults to one.
#' @keywords degrees of freedom
#' @export
#' @examples
#' # selects the columns containing a string that identifies the group
#' group1 <- grep("Sample_norm", colnames(data))
#' # three analytical replicates were run for each
#' data$group1_df <- count_df(data, group1, rep = 3)
count_df <- function (dat, col, rep = 1){
list <- NULL
for (i in 1:nrow(dat)){
temp <- length(which(!is.na(dat[i, col])))
temp <- temp / rep
if(temp >= 1){
temp <- temp - 1
}
list <- c(list, temp)
}
return(list)
}
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