Mass cytometry (CyTOF) uses heavy metal isotopes rather than fluorescent tags as reporters to label antibodies, thereby substantially decreasing spectral overlap and allowing for examination of over 50 parameters at the single cell level. While spectral overlap is significantly less pronounced in CyTOF than flow cytometry, spillover due to detection sensitivity, isotopic impurities, and oxide formation can impede data interpretability.
We designed CATALYST (Cytometry dATa anALYSis Tools) to provide:
There are various entry points to using the CATALYST tools. If you are an R user, we recommend using the functions from the R package itself, from the command line or via a script. If you want to use the graphical environment, we recommend to run it locally on your own computer, as this will be faster and in terms of dataset sizes, is only limited by the resources on your computer. To do this, you will need to install recent versions of R and the necessary R packages (details below). If installing all the necessary R packages is a hurdle, you can use the hosted version.
For detailed examples and usage instructions, see the package vignettes under Articels.
Using the GUI
CATALYST::launchGUI()inside an R session.
CATALYST is still under active development. We greatly welcome (and highly encourage!) all feedback, bug reports and suggestions for improvement. Please make sure to raise issues with a reproducible example and, if you're running R, the output of your
Any scripts or data that you put into this service are public.
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.