CATALYST: Cytometry dATa anALYSis Tools

Mass cytometry (CyTOF) uses heavy metal isotopes rather than fluorescent tags as reporters to label antibodies, thereby substantially decreasing spectral overlap and allowing for examination of over 50 parameters at the single cell level. While spectral overlap is significantly less pronounced in CyTOF than flow cytometry, spillover due to detection sensitivity, isotopic impurities, and oxide formation can impede data interpretability. We designed CATALYST (Cytometry dATa anALYSis Tools) to provide a pipeline for preprocessing of cytometry data, including i) normalization using bead standards, ii) single-cell deconvolution, and iii) bead-based compensation.

Package details

AuthorHelena L. Crowell [aut, cre], Vito R.T. Zanotelli [aut], Stéphane Chevrier [aut, dtc], Mark D. Robinson [aut, fnd], Bernd Bodenmiller [fnd]
Bioconductor views DifferentialExpression FlowCytometry ImmunoOncology MassSpectrometry Normalization Preprocessing SingleCell Software StatisticalMethod Visualization
MaintainerHelena L. Crowell <[email protected]>
LicenseGPL (>=2)
Package repositoryView on Bioconductor
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CATALYST documentation built on June 13, 2019, 2 a.m.