Description Usage Arguments Value
View source: R/internal_fluo_est.R
It produces a table of estimated spot locations and fluorescence signals accompanied by informative plots. It can process the results of either spotCoords() for fluorescence-based estimation or forceBF() for BF image modelling estimation.
1 2 |
img |
Data matrix. The original data of the BF image to be read and processed. |
chaImgs |
List. A list of the channel images (data matrices) of a sample. |
binChaImgs |
List. A list of binary segmented channel image data. |
center |
Data matrix. The 2-dimensional location of the spot's representative center. |
BFcoords |
List. A list of statistics describing the C1 chip line patterns (for BF image modelling). |
BFarea |
Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated. This is mainly used in BF image modeling where a fluorescence spot could not be originally detected. The value of this parameter is also used as a cut-off to find matched spots across channel of the same sample image. |
log.transform |
Logical. If TRUE the image data are plotted in the log scale. |
warning |
Character string. An indicator of the estimation type that has been internally performed, i.e. fluorescence-based or BF image modelling. |
minDiff |
Float. the mu_hat of the H0: image-to-noise ratio = log(foreground_signal) - log(background_signal) = mu_hat. Rejection of H0 implies that the identified spot is brighter than background. Default is 0.5. |
separator |
Character string. Removes the Bright Field ("BF") and channel indicators from the image file names (see <<separator2>> in readFiles()). |
image.type |
Character string. A triplet of IDs to characterize the type of images under study. They refer to the ImageType part of the original image or txt file names. |
A table of location and fluorescence estimated with accompanied plots
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