Description Usage Arguments Value
View source: R/internal_fluo_est.R
It estimates the spot area by joining the red and green bright spot location estimates.
1 |
areaR |
Data matrix. The bright (spot) coordinates in one channel. |
areaG |
Data matrix. The bright (spot) coordinates in the other channel. |
center |
Data matrix. The 2-dimensional location of the spot's center. |
areaBased |
Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated. |
warning |
Character string. An indicator of the estimation type that has been internally performed, i.e. fluorescence-based or BF image modelling. |
chaImgs |
Data matrix. The channel binary segmented image data that are used for reference to obtain the area of interest. |
The coordinates of the spot area and its length in pixels.
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