spotCenter: spotCenter

Description Usage Arguments Value

View source: R/internal_fluo_est.R

Description

It estimates a series of spot statistics on each channel.

Usage

1
spotCenter(img, foregroundCut, howbig, ImgLimits)

Arguments

img

Data matrix. The channel image data.

foregroundCut

Numeric vector. The binary segmentation image analysis cutoffs for normalized image data. Pixels with normalized signals higher than the cutoff belong to foreground.

howbig

Integer. An user defined value of the minimum expected spot size.

ImgLimits

a cutoff that determines where the spot is supposed to be found.

Value

A list of spot coordinate estimates


CONFESS documentation built on May 2, 2018, 2:32 a.m.