straightColLines: straightColLines

In CONFESS: Cell OrderiNg by FluorEScence Signal

Description

It identifies the vertical BF image characteristic lines.

Usage

straightColLines(img, pattern.search, cut = seq(0.3, 1.5, 0.02),
  ImgLimits, chip.type)

Arguments

img	Data matrix. The BF image data matrix.
pattern.search	Integer. A cutoff to find horizontal and vertical lines on the chip.
cut	Integer. A normalized signal cutoff above which a pixel is considered as foreground.
ImgLimits	Integer. It instructs the algorithm to find spots in a certain central image area. For example, for a 512 x 512 image with cutSides = 50, it will search for spots in the central area [ImgLimits:(512-ImgLimits),ImgLimits:(512-ImgLimits)] of the image matrix.
chip.type	Character string. It specifies the type of Fluidigm chip to be analyzed.

Value

Estimated vertical BF characteristic lines.

CONFESS documentation built on Nov. 8, 2020, 6:05 p.m.