Description Usage Arguments Value
View source: R/internal_fluo_est.R
It identifies the horizontal BF image characteristic lines.
1 | straightRowLines(colData, pattern.search, ImgLimits)
|
colData |
List. The data to be analyzed. |
pattern.search |
Integer. A cutoff to find horizontal and vertical lines on the chip |
ImgLimits |
Integer. It instructs the algorithm to find spots in a certain central image area. For example, for a 512 x 512 image with cutSides = 50, it will search for spots in the central area [ImgLimits:(512-ImgLimits),ImgLimits:(512-ImgLimits)] of the image matrix. |
The estimated horizontal BF characteristic lines
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