BrowseSeqs: View Sequences in a Web Browser

Description Usage Arguments Details Value Author(s) References See Also Examples

View source: R/BrowseSeqs.R

Description

Opens an html file in a web browser to show the sequences in an XStringSet.

Usage

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BrowseSeqs(myXStringSet,
           htmlFile = paste(tempdir(), "/myXStringSet.html", sep = ""),
           openURL = interactive(),
           colorPatterns = TRUE,
           highlight = NA,
           patterns = c("-", alphabet(myXStringSet, baseOnly=TRUE)),
           colors = substring(rainbow(length(patterns),
                              v=0.8, start=0.9, end=0.7), 1, 7),
           colWidth = Inf,
           ...)

Arguments

myXStringSet

A XStringSet object of sequences.

htmlFile

Character string giving the location where the html file should be written.

openURL

Logical indicating whether the htmlFile should be opened in a web browser.

colorPatterns

Logical specifying whether to color matched patterns, or an integer vector providing pairs of start and stop boundaries for coloring.

highlight

Numeric specifying which sequence in the set to use for comparison or NA to color all sequences (default). If highlight is 0 then positions differing from the consensus sequence are highlighted.

patterns

Either an AAStringSet, DNAStringSet, or RNAStringSet object, a character vector containing regular expressions, a list of numeric matrices, or NULL. (See details section below.)

colors

Character vector providing the color for each of the matched patterns. Typically a character vector with elements of 7 characters: “#” followed by the red, blue, green values in hexadecimal (after rescaling to 0 ... 255). Ignored when patterns is a list of matrices.

colWidth

Integer giving the maximum number of nucleotides wide the display can be before starting a new page. Must be a multiple of 20 (e.g., 100), or Inf (the default) to display all the sequences in one set of rows.

...

Additional arguments to adjust the appearance of the consensus sequence at the base of the display. Passed directly to ConsensusSequence for an AAStringSet, DNAStringSet, or RNAStringSet, or to consensusString for a BStringSet.

Details

BrowseSeqs converts an XStringSet into html format for viewing in a web browser. The sequences are colored in accordance with the patterns that are provided, or left uncolored if colorPatterns is FALSE or patterns is NULL. Character or XStringSet patterns are matched as regular expressions and colored according to colors. If patterns is a list of matrices, then it must contain one element per sequence. Each matrix is interpreted as providing the fraction red, blue, and green for each letter in the sequence. Thus, colors is ignored when patterns is a list. (See examples section below.)

Patterns are not matched across column breaks, so multi-character patterns should be carefully considered when colWidth is less than the maximum sequence length. Patterns are matched sequentially in the order provided, so it is feasible to use nested patterns such as c("ACCTG", "CC"). In this case the “CC” could be colored differently inside the previously colored “ACCTG”. Note that patterns overlapping the boundaries of a previously matched pattern will not be matched. For example, “ACCTG” would not be matched if patterns=c("CC", "ACCTG").

Some web browsers cannot quickly display a large amount colored text, so it is recommended to use colorPatterns = FALSE or to highlight a sequence when viewing a large XStringSet. Highlighting will only show all of the characters in the highlighted sequence, and convert all matching positions in the other sequences into dots without color. Also, note that some web browsers display small shifts between fixed-width characters that may become noticeable as color offsets between the ends of long sequences.

Value

Creates an html file containing sequence data and (if openURL is TRUE) opens it in a web browser for viewing. The layout has the sequence name on the left, position legend on the top, cumulative number of nucleotides on the right, and consensus sequence on the bottom.

Returns htmlFile if the html file was written successfully.

Author(s)

Erik Wright [email protected]

References

ES Wright (2016) "Using DECIPHER v2.0 to Analyze Big Biological Sequence Data in R". The R Journal, 8(1), 352-359.

See Also

BrowseDB, ConsensusSequence

Examples

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# load the example DNA sequences
db <- system.file("extdata", "Bacteria_175seqs.sqlite", package="DECIPHER")
dna <- SearchDB(db) # non-coding ribosomal RNA gene sequences

# example of using the defaults with DNA sequences
BrowseSeqs(dna) # view the XStringSet

# color only "ACTG" and "CSC" patterns (where S is C or G)
BrowseSeqs(dna, patterns=DNAStringSet(c("ACTG", "CSC")))

# highlight (i.e., only fully-color) the first sequence
BrowseSeqs(dna, highlight=1) # other sequences are dots where matching

# highlight the consensus sequence at the bottom
BrowseSeqs(dna, highlight=0) # other sequences are dots where matching

# split the wide view into multiple vertical pages (i.e., for printing)
BrowseSeqs(dna, colWidth=100, highlight=1)

# specify an alternative color scheme for -, A, C, G, T
BrowseSeqs(dna, colors=c("#1E90FF", "#32CD32", "#9400D3", "black", "#EE3300"))

# only color the positions within certain positional ranges (100-200 & 250-500)
BrowseSeqs(dna, colorPatterns=c(100, 200, 250, 500))

# color according to base-pairing by supplying the fraction RGB for every position
dbn <- PredictDBN(dna, type="structures") # calculate the secondary structures
# dbn now contains the scores for whether a base is paired (left/right) or unpaired
dbn[[1]][, 1] # the scores for the first position in the first sequence
dbn[[2]][, 10] # the scores for the tenth position in the second sequence
# these positional scores can be used as shades of red, green, and blue:
BrowseSeqs(dna, patterns=dbn) # red = unpaired, green = left-pairing, blue = right
# positions in black are not part of the consensus secondary structure

# color all restriction sites
data(RESTRICTION_ENZYMES) # load dataset containing restriction enzyme sequences
sites <- RESTRICTION_ENZYMES
sites <- gsub("[^A-Z]", "", sites) # remove non-letters
sites <- DNAStringSet(sites) # convert the character vector to a DNAStringSet
rc_sites <- reverseComplement(DNAStringSet(sites))
w <- which(sites != rc_sites) # find non-palindromic restriction sites
sites <- c(sites, rc_sites[w]) # append their reverse complement
sites <- sites[order(nchar(sites))] # match shorter sites first
BrowseSeqs(dna, patterns=sites)

# load the example protein coding sequences
fas <- system.file("extdata", "50S_ribosomal_protein_L2.fas", package="DECIPHER")
dna <- readDNAStringSet(fas)

# example of using the defaults with amino acid sequences
aa <- unique(translate(dna)) # the unique amino acid sequences
BrowseSeqs(aa)

# example of highlighting the consensus amino acid sequence
AA <- AlignSeqs(aa)
BrowseSeqs(AA, highlight=0)

# example of highlighting positions that differ from the majority consensus
BrowseSeqs(AA, highlight=0, threshold=0.5)

# color amino acids according to their predicted secondary structure
hec <- PredictHEC(AA, type="probabilities") # calculate the secondary structures
# hec now contains the probability that a base is in an alpha-helix or beta-sheet
hec[[3]][, 18] # the 18th position in sequence 3 is likely part of a beta-sheet (E)
# the positional probabilities can be used as shades of red, green, and blue:
BrowseSeqs(AA, patterns=hec) # red = alpha-helix, green = beta-sheet, blue = coil

DECIPHER documentation built on Nov. 17, 2017, 1:01 p.m.