Mask Highly Variable Regions of An Alignment
Automatically masks poorly aligned regions of an alignment based on sequence conservation and gap frequency.
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Integer value specifying the size of the region to the left and right of the center-point to use in calculating the moving average.
Numeric giving the average entropy in bits from 0 to 2 below which a region is masked.
Numeric specifying the maximum faction of gaps in an alignment column to be masked.
Logical specifying whether or not to show a plot of the positions that were kept or masked.
Poorly aligned regions of a multiple sequence alignment may lead to incorrect results in downstream analyses, and require extra processing time. One method to mitigate their effects is to mask columns of the alignment that may be poorly aligned, such as highly-variable regions or regions with many insertions and deletions (gaps).
Highly variable regions are detected by their signature of having low information content. A moving average of
windowSize nucleotides to the left and right of the center-point is applied to smooth noise in the information content signal along the sequence. Regions dropping below
threshold bits or more than
maxFractionGaps are masked in the returned alignment.
MultipleAlignment object of the input type with masked columns where the input criteria are met.
Erik Wright DECIPHER@cae.wisc.edu
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fas <- system.file("extdata", "Streptomyces_ITS_aligned.fas", package="DECIPHER") dna <- readDNAStringSet(fas) masked_dna <- MaskAlignment(dna, showPlot=TRUE) # display only unmasked nucleotides for use in downstream analyses not_masked <- as(masked_dna, "DNAStringSet") BrowseSeqs(not_masked) # display only masked nucleotides that are covered by the mask masked <- masked_dna colmask(masked, append="replace", invert=TRUE) <- colmask(masked) masked <- as(masked, "DNAStringSet") BrowseSeqs(masked) # display the complete DNA sequence set including the mask masks <- lapply(width(colmask(masked_dna)), rep, x="+") masks <- unlist(lapply(masks, paste, collapse="")) masked_dna <- replaceAt(dna, at=IRanges(colmask(masked_dna)), value=masks) BrowseSeqs(masked_dna)
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