qPCRraw: Example raw qPCR data.

In HTqPCR: Automated analysis of high-throughput qPCR data

Description

Six qPCR samples, performed on the TaqMan Low Density Arrays from Applied Biosystem. Each sample contains 384 PCR reactions, and there are 3 different samples with 2 replicates each. To be used as example data in the HTqPCR package.

Usage

data(qPCRraw)

Format

The format is: Formal class 'qPCRset' [package ".GlobalEnv"] with 8 slots ..@ CtHistory :'data.frame': 1 obs. of 1 variable: .. ..$ history: chr "readCtData(files = exFiles$File, path = exPath)" ..@ assayData :<environment: 0x118094e30> ..@ phenoData :Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots .. .. ..@ varMetadata :'data.frame': 1 obs. of 1 variable: .. .. .. ..$ labelDescription: chr "Sample numbering" .. .. ..@ data :'data.frame': 6 obs. of 1 variable: .. .. .. ..$ sample: int [1:6] 1 2 3 4 5 6 .. .. ..@ dimLabels : chr [1:2] "sampleNames" "sampleColumns" .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots .. .. .. .. ..@ .Data:List of 1 .. .. .. .. .. ..$ : int [1:3] 1 1 0 ..@ featureData :Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots .. .. ..@ varMetadata :'data.frame': 4 obs. of 1 variable: .. .. .. ..$ labelDescription: chr [1:4] NA NA NA NA .. .. ..@ data :'data.frame': 384 obs. of 4 variables: .. .. .. ..$ featureNames: Factor w/ 191 levels "Gene1","Gene10",..: 1 104 115 126 137 148 159 170 181 2 ... .. .. .. ..$ featureType : Factor w/ 2 levels "Endogenous Control",..: 1 2 2 2 2 2 2 2 2 2 ... .. .. .. ..$ featurePos : Factor w/ 384 levels "A1","A10","A11",..: 1 12 18 19 20 21 22 23 24 2 ... .. .. .. ..$ featureClass: Factor w/ 3 levels "Kinase","Marker",..: 1 2 1 3 2 2 2 3 1 2 ... .. .. ..@ dimLabels : chr [1:2] "featureNames" "featureColumns" .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots .. .. .. .. ..@ .Data:List of 1 .. .. .. .. .. ..$ : int [1:3] 1 1 0 ..@ experimentData :Formal class 'MIAME' [package "Biobase"] with 13 slots .. .. ..@ name : chr "" .. .. ..@ lab : chr "" .. .. ..@ contact : chr "" .. .. ..@ title : chr "" .. .. ..@ abstract : chr "" .. .. ..@ url : chr "" .. .. ..@ pubMedIds : chr "" .. .. ..@ samples : list() .. .. ..@ hybridizations : list() .. .. ..@ normControls : list() .. .. ..@ preprocessing : list() .. .. ..@ other : list() .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots .. .. .. .. ..@ .Data:List of 2 .. .. .. .. .. ..$ : int [1:3] 1 0 0 .. .. .. .. .. ..$ : int [1:3] 1 1 0 ..@ annotation : chr(0) ..@ protocolData :Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots .. .. ..@ varMetadata :'data.frame': 0 obs. of 1 variable: .. .. .. ..$ labelDescription: chr(0) .. .. ..@ data :'data.frame': 6 obs. of 0 variables .. .. ..@ dimLabels : chr [1:2] "sampleNames" "sampleColumns" .. .. ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots .. .. .. .. ..@ .Data:List of 1 .. .. .. .. .. ..$ : int [1:3] 1 1 0 ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots .. .. ..@ .Data:List of 3 .. .. .. ..$ : int [1:3] 2 14 0 .. .. .. ..$ : int [1:3] 2 14 0 .. .. .. ..$ : int [1:3] 1 3 0

Examples

data(qPCRraw)

Example output

Loading required package: Biobase
Loading required package: BiocGenerics
Loading required package: parallel

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:parallel':

    clusterApply, clusterApplyLB, clusterCall, clusterEvalQ,
    clusterExport, clusterMap, parApply, parCapply, parLapply,
    parLapplyLB, parRapply, parSapply, parSapplyLB

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, append,
    as.data.frame, cbind, colMeans, colSums, colnames, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, lengths, mapply, match, mget, order, paste, pmax, pmax.int,
    pmin, pmin.int, rank, rbind, rowMeans, rowSums, rownames, sapply,
    setdiff, sort, table, tapply, union, unique, unsplit, which,
    which.max, which.min

Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.

Loading required package: RColorBrewer
Loading required package: limma

Attaching package: 'limma'

The following object is masked from 'package:BiocGenerics':

    plotMA

Warning message:
In read.dcf(con) :
  URL 'http://bioconductor.org/BiocInstaller.dcf': status was 'Couldn't resolve host name'

HTqPCR documentation built on Nov. 8, 2020, 6:51 p.m.