Description Objects from the Class Slots Extends Methods Note Author(s) References See Also Examples

ratio transform calculates the ratio of two parameters defined by the function

*f(parameter_1,parameter_2)=\frac{parameter_1}{parameter_2}*

Objects can be created by calls to the constructor
```
ratio(parameter1,parameter2,transformationId)
```

.

`.Data`

:Object of class

`"function"`

~~`numerator`

:Object of class

`"transformation"`

-flow parameter to be transformed`denominator`

:Object of class

`"transformation"`

-flow parameter to be transformed`transformationId`

:Object of class

`"character"`

unique ID to reference the transformation

Class `"transform"`

, directly.
Class `"transformation"`

, by class "transform", distance 2.
Class `"characterOrTransformation"`

, by class "transform", distance 3.

No methods defined with class "ratio" in the signature.

The ratio transformation object can be evaluated using the eval method by passing the data frame as an argument.The transformed parameters are returned as matrix with one column. (See example below)

Gopalakrishnan N, F.Hahne

Gating-ML Candidate Recommendation for Gating Description in Flow Cytometry V 1.5

dg1polynomial,quadratic,squareroot

1 2 3 4 | ```
dat <- read.FCS(system.file("extdata","0877408774.B08",
package="flowCore"))
rat1<-ratio("FSC-H","SSC-H",transformationId="rat1")
transOut<-eval(rat1)(exprs(dat))
``` |

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