Description Usage Arguments Details Value References See Also Examples
View source: R/TrinucleotideMatrix.R
Extract single 5' and 3' bases flanking the mutated site for de-novo signature analysis. Also estimates APOBEC enrichment scores.
1 2 3 4 5 6 7 8 9 |
maf |
an |
ref_genome |
BSgenome object or name of the installed BSgenome package. Example: BSgenome.Hsapiens.UCSC.hg19 Default NULL, tries to auto-detect from installed genomes. |
prefix |
Prefix to add or remove from contig names in MAF file. |
add |
If prefix is used, default is to add prefix to contig names in MAF file. If false prefix will be removed from contig names. |
ignoreChr |
Chromsomes to ignore from analysis. e.g. chrM |
useSyn |
Logical. Whether to include synonymous variants in analysis. Defaults to TRUE |
fn |
If given writes APOBEC results to an output file with basename fn. Default NULL. |
Extracts immediate 5' and 3' bases flanking the mutated site and classifies them into 96 substitution classes. Requires BSgenome data packages for sequence extraction.
APOBEC Enrichment: Enrichment score is calculated using the same method described by Roberts et al.
E = (n_tcw * background_c) / (n_C * background_tcw)
where, n_tcw = number of mutations within T[C>T]W and T[C>G]W context. (W -> A or T)
n_C = number of mutated C and G
background_C and background_tcw motifs are number of C and TCW motifs occuring around +/- 20bp of each mutation.
One-sided Fisher's Exact test is performed to determine the enrichment of APOBEC tcw mutations over background.
list of 2. A matrix of dimension nx96, where n is the number of samples in the MAF and a table describing APOBEC enrichment per sample.
Roberts SA, Lawrence MS, Klimczak LJ, et al. An APOBEC Cytidine Deaminase Mutagenesis Pattern is Widespread in Human Cancers. Nature genetics. 2013;45(9):970-976. doi:10.1038/ng.2702.
extractSignatures
plotApobecDiff
1 2 3 4 5 6 7 | ## Not run:
laml.maf <- system.file("extdata", "tcga_laml.maf.gz", package = "maftools")
laml <- read.maf(maf = laml.maf)
laml.tnm <- trinucleotideMatrix(maf = laml, ref_genome = 'BSgenome.Hsapiens.UCSC.hg19',
prefix = 'chr', add = TRUE, useSyn = TRUE)
## End(Not run)
|
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.