score.calc.epistasis.LR.MC: Calculate -log10(p) of epistatic effects by LR test... In RAINBOWR: Genome-Wide Association Study with SNP-Set Methods

Description

Calculate -log10(p) of epistatic effects by LR test (multi-cores)

Usage

  1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 score.calc.epistasis.LR.MC( M.now, y, X.now, ZETA.now, package.MM = "gaston", eigen.SGS = NULL, eigen.G = NULL, n.core = 2, parallel.method = "mclapply", optimizer = "nlminb", map, haplotype = TRUE, num.hap = NULL, window.size.half = 5, window.slide = 1, chi0.mixture = 0.5, gene.set = NULL, dominance.eff = TRUE, skip.self.int = FALSE, min.MAF = 0.02, count = TRUE ) 

Arguments

 M.now A n \times m genotype matrix where n is sample size and m is the number of markers. y A n \times 1 vector. A vector of phenotypic values should be used. NA is allowed. X.now A n \times p matrix. You should assign mean vector (rep(1, n)) and covariates. NA is not allowed. ZETA.now A list of variance (relationship) matrix (K; m \times m) and its design matrix (Z; n \times m) of random effects. You can use only one kernel matrix. For example, ZETA = list(A = list(Z = Z, K = K)) Please set names of list "Z" and "K"! package.MM The package name to be used when solving mixed-effects model. We only offer the following three packages: "RAINBOWR", "MM4LMM" and "gaston". Default package is 'gaston'. See more details at EM3.general. eigen.SGS A list with $valuesEigen values$vectorsEigen vectors The result of the eigen decompsition of SGS, where S = I - X(X'X)^{-1}X', G = ZKZ'. You can use "spectralG.cpp" function in RAINBOWR. If this argument is NULL, the eigen decomposition will be performed in this function. We recommend you assign the result of the eigen decomposition beforehand for time saving. eigen.G A list with $valuesEigen values$vectorsEigen vectors The result of the eigen decompsition of G = ZKZ'. You can use "spectralG.cpp" function in RAINBOWR. If this argument is NULL, the eigen decomposition will be performed in this function. We recommend you assign the result of the eigen decomposition beforehand for time saving. n.core Setting n.core > 1 will enable parallel execution on a machine with multiple cores. This argument is not valid when 'parallel.method = "furrr"'. parallel.method Method for parallel computation. We offer three methods, "mclapply", "furrr", and "foreach". When 'parallel.method = "mclapply"', we utilize pbmclapply function in the 'pbmcapply' package with 'count = TRUE' and mclapply function in the 'parallel' package with 'count = FALSE'. When 'parallel.method = "furrr"', we utilize future_map function in the 'furrr' package. With 'count = TRUE', we also utilize progressor function in the 'progressr' package to show the progress bar, so please install the 'progressr' package from github (https://github.com/HenrikBengtsson/progressr). For 'parallel.method = "furrr"', you can perform multi-thread parallelization by sharing memories, which results in saving your memory, but quite slower compared to 'parallel.method = "mclapply"'. When 'parallel.method = "foreach"', we utilize foreach function in the 'foreach' package with the utilization of makeCluster function in 'parallel' package, and registerDoParallel function in 'doParallel' package. With 'count = TRUE', we also utilize setTxtProgressBar and txtProgressBar functions in the 'utils' package to show the progress bar. We recommend that you use the option 'parallel.method = "mclapply"', but for Windows users, this parallelization method is not supported. So, if you are Windows user, we recommend that you use the option 'parallel.method = "foreach"'. optimizer The function used in the optimization process. We offer "optim", "optimx", and "nlminb" functions. map Data frame of map information where the first column is the marker names, the second and third column is the chromosome amd map position, and the forth column is -log10(p) for each marker. haplotype If the number of lines of your data is large (maybe > 100), you should set haplotype = TRUE. When haplotype = TRUE, haplotype-based kernel will be used for calculating -log10(p). (So the dimension of this gram matrix will be smaller.) The result won't be changed, but the time for the calculation will be shorter. num.hap When haplotype = TRUE, you can set the number of haplotypes which you expect. Then similar arrays are considered as the same haplotype, and then make kernel(K.SNP) whose dimension is num.hap x num.hap. When num.hap = NULL (default), num.hap will be set as the maximum number which reflects the difference between lines. window.size.half This argument decides how many SNPs (around the SNP you want to test) are used to calculated K.SNP. More precisely, the number of SNPs will be 2 * window.size.half + 1. window.slide This argument determines how often you test markers. If window.slide = 1, every marker will be tested. If you want to perform SNP set by bins, please set window.slide = 2 * window.size.half + 1. chi0.mixture RAINBOWR assumes the tdeviance is considered to follow a x chisq(df = 0) + (1 - a) x chisq(df = r). where r is the degree of freedom. The argument chi0.mixture is a (0 <= a < 1), and default is 0.5. gene.set If you have information of gene, you can use it to perform kernel-based GWAS. You should assign your gene information to gene.set in the form of a "data.frame" (whose dimension is (the number of gene) x 2). In the first column, you should assign the gene name. And in the second column, you should assign the names of each marker, which correspond to the marker names of "geno" argument. dominance.eff If this argument is TRUE, dominance effect is included in the model, and additive x dominance and dominance x dominance are also tested as epistatic effects. When you use inbred lines, please set this argument FALSE. skip.self.int As default, the function also tests the self-interactions among the same SNP-sets. If you want to avoid this, please set 'skip.self.int = TRUE'. min.MAF Specifies the minimum minor allele frequency (MAF). If a marker has a MAF less than min.MAF, it is assigned a zero score. count When count is TRUE, you can know how far RGWAS has ended with percent display.

Value

-log10(p) of epistatic effects for each SNP-set

References

Listgarten, J. et al. (2013) A powerful and efficient set test for genetic markers that handles confounders. Bioinformatics. 29(12): 1526-1533.

Lippert, C. et al. (2014) Greater power and computational efficiency for kernel-based association testing of sets of genetic variants. Bioinformatics. 30(22): 3206-3214.

Jiang, Y. and Reif, J.C. (2015) Modeling epistasis in genomic selection. Genetics. 201(2): 759-768.

RAINBOWR documentation built on Jan. 7, 2022, 5:15 p.m.