inst/supp_doc2/supp_doc2.md
In GranderLab/miR34a_asRNA_project: The miR34AasRNAproject package facilitates the transparency and reproducibility of the miR34a asRNA project and associated publication.
Supplementary Document 2
Figure 1b
| name | sequence |
| ---------------- |:--------------------------------:|
| miR34a asRNA F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| miR34a asRNA R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
| miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA |
| miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT |
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Figure 1d
| name | sequence |
| ----------------- |:---------------------------------------------------------------------------------------------------:|
| miR34a asRNA F10 | ACG CGT CTC TCC AGC CCG GGA T |
| polyT T7 FAM | CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG ACA TCC GTA GCT CGT CCA GGA CCC TTT TTT TTT TTT TTT TTT VN |
| miR34a asRNA F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| FAM primer | CCG TAG CTC GTC CAG GAC CC |
Figure 2a
| name | sequence |
| ------------- |:--------------------------------:|
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TCG GGA TGT CCA CGT CA |
| miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA |
| miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT |
| miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 2b
| name | sequence |
| ------------- |:--------------------------------:|
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TCG GGA TGT CCA CGT CA |
| miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA |
| miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT |
| miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 2c
QPCR primers
| name | sequence |
| ------------- |:--------------------------------:|
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TCG GGA TGT CCA CGT CA |
| miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA |
| miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT |
| miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 2d
| name | sequence |
| ------------------ |:-------------------------------:|
| Luc setII F | AAG ATT CAA AGT GCG CTG CTG |
| Luc setII R | TTG CCT GAT ACC TGG CAG ATG |
| Renilla pBiDir F1 | TAA CGC GGC CTC TTC TTA TTT |
| Renilla pBiDir R1 | GAT TTG CCT GAT TTG CCC ATA |
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Figure 2e
| name | sequence |
| ------------------ |:-------------------------------:|
| Luc setII F | AAG ATT CAA AGT GCG CTG CTG |
| Luc setII R | TTG CCT GAT ACC TGG CAG ATG |
| Renilla pBiDir F1 | TAA CGC GGC CTC TTC TTA TTT |
| Renilla pBiDir R1 | GAT TTG CCT GAT TTG CCC ATA |
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Figure 3a
Cloning primers
| name | sequence |
| ----------------------- |:--------------------------------:|
| miR34aAS cloning F4 | ACG CGT CTC TCC AGC CCG GGA T |
| miR34aAS cloning Ex3_1 | AAT GAT GGC CGC AAC TAA TGA CGG |
QPCR primers
| name | sequence |
| ------------ |:--------------------------------:|
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TCG GGA TGT CCA CGT CA |
| miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 3d
| name | sequence |
| --------------- |:--------------------------------:|
| miR34a ChIP F1 | AAA GTT TGC AAA GAA GGA GGC GGG |
| miR34a ChIP R1 | AGG GAA GAA AGA ACT AGC CGA GCA |
Supplementary Figure 2a
| name | sequence |
| -------------- |:--------------------------------:|
| miR34a AS F10 | ACG CGT CTC TCC AGC CCG GGA T |
| miR34a AS F11 | ATC TGC GTG GTC ACC GAG AAG CA |
| miR34a AS F12 | CGC ACG GAC TGA GAA ACA CAA G |
| miR34a AS F13 | ACG GAG GCT ACA CAA TTG AAC AGG |
| miR34a AS F14 | AGG GAA GAA AGA ACT AGC CGA GCA |
| miR34a AS F15 | CAT TTG CTG CAA TAT CAC CGT GGC |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Supplementary Figure 2b
| name | sequence |
| ------------------ |:--------------------------------:|
| miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
| miR34a AS int1 R1 | TGC GCA AAC TAC GCG CTC T |
| miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA |
| miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT |
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
| U48 F | AGT GAT GAT GAC CCC AGG TA |
| U48 R | GGT CAG AGC GCT GCG GTG AT |
Supplementary Figure 2c
| name | sequence |
| ----------------- |:--------------------------------:|
| miR34a AS F12 | AAA CAC AAG CGT TTA CCT GGG TGC |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
| miR34a AS R2 | ATA GGT TCA TTT GCC CGA TGT GCC |
| miR34a AS R3 | CCA CAG CTG TTG CTT CTG AAT GCT |
| miR34a AS Ex3 R1 | TGA TGG CCG CAA CTA ATG ACG GAT |
Supplementary Figure 3c
| name | sequence |
| ------------------ |:-------------------------------:|
| Luc setII F | AAG ATT CAA AGT GCG CTG CTG |
| Luc setII R | TTG CCT GAT ACC TGG CAG ATG |
| Renilla pBiDir F1 | TAA CGC GGC CTC TTC TTA TTT |
| Renilla pBiDir R1 | GAT TTG CCT GAT TTG CCC ATA |
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Supplementary Figure 4a
Cloning primers
| name | sequence |
| ----------------------- |:--------------------------------:|
| miR34aAS cloning F4 | ACG CGT CTC TCC AGC CCG GGA T |
| miR34aAS cloning Ex3_1 | AAT GAT GGC CGC AAC TAA TGA CGG |
QPCR primers
| name | sequence |
| ------------- |:--------------------------------:|
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TCG GGA TGT CCA CGT CA |
| miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA |
| miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Supplementary Figure 4c
| name | sequence |
| ------------ |:-------------------------------:|
| CCND1 Fwd | CGT GGC CTC TAA GAT GAA GG |
| CCND1 Rev | CTG GCA TTT TGG AGA GGA AG |
| ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG |
| ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
GranderLab/miR34a_asRNA_project documentation built on May 26, 2019, 7:26 a.m.
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Supplementary Document 2
Figure 1b
| name | sequence | | ---------------- |:--------------------------------:| | miR34a asRNA F1 | AGC GGC ATC TCC TCC ACC TGA AA | | miR34a asRNA R1 | TTG CCT CGT GAG TCC AAG GAG AAT | | miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA | | miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT | | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Figure 1d
| name | sequence | | ----------------- |:---------------------------------------------------------------------------------------------------:| | miR34a asRNA F10 | ACG CGT CTC TCC AGC CCG GGA T | | polyT T7 FAM | CAG TGA ATT GTA ATA CGA CTC ACT ATA GGG ACA TCC GTA GCT CGT CCA GGA CCC TTT TTT TTT TTT TTT TTT VN | | miR34a asRNA F1 | AGC GGC ATC TCC TCC ACC TGA AA | | FAM primer | CCG TAG CTC GTC CAG GAC CC |
Figure 2a
| name | sequence | | ------------- |:--------------------------------:| | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TCG GGA TGT CCA CGT CA | | miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA | | miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT | | miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 2b
| name | sequence | | ------------- |:--------------------------------:| | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TCG GGA TGT CCA CGT CA | | miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA | | miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT | | miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 2c
QPCR primers
| name | sequence | | ------------- |:--------------------------------:| | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TCG GGA TGT CCA CGT CA | | miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA | | miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT | | miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 2d
| name | sequence | | ------------------ |:-------------------------------:| | Luc setII F | AAG ATT CAA AGT GCG CTG CTG | | Luc setII R | TTG CCT GAT ACC TGG CAG ATG | | Renilla pBiDir F1 | TAA CGC GGC CTC TTC TTA TTT | | Renilla pBiDir R1 | GAT TTG CCT GAT TTG CCC ATA | | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Figure 2e
| name | sequence | | ------------------ |:-------------------------------:| | Luc setII F | AAG ATT CAA AGT GCG CTG CTG | | Luc setII R | TTG CCT GAT ACC TGG CAG ATG | | Renilla pBiDir F1 | TAA CGC GGC CTC TTC TTA TTT | | Renilla pBiDir R1 | GAT TTG CCT GAT TTG CCC ATA | | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Figure 3a
Cloning primers
| name | sequence | | ----------------------- |:--------------------------------:| | miR34aAS cloning F4 | ACG CGT CTC TCC AGC CCG GGA T | | miR34aAS cloning Ex3_1 | AAT GAT GGC CGC AAC TAA TGA CGG |
QPCR primers
| name | sequence | | ------------ |:--------------------------------:| | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TCG GGA TGT CCA CGT CA | | miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Figure 3d
| name | sequence | | --------------- |:--------------------------------:| | miR34a ChIP F1 | AAA GTT TGC AAA GAA GGA GGC GGG | | miR34a ChIP R1 | AGG GAA GAA AGA ACT AGC CGA GCA |
Supplementary Figure 2a
| name | sequence | | -------------- |:--------------------------------:| | miR34a AS F10 | ACG CGT CTC TCC AGC CCG GGA T | | miR34a AS F11 | ATC TGC GTG GTC ACC GAG AAG CA | | miR34a AS F12 | CGC ACG GAC TGA GAA ACA CAA G | | miR34a AS F13 | ACG GAG GCT ACA CAA TTG AAC AGG | | miR34a AS F14 | AGG GAA GAA AGA ACT AGC CGA GCA | | miR34a AS F15 | CAT TTG CTG CAA TAT CAC CGT GGC | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Supplementary Figure 2b
| name | sequence | | ------------------ |:--------------------------------:| | miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT | | miR34a AS int1 R1 | TGC GCA AAC TAC GCG CTC T | | miR34a HG F | TCT GCT CCA GTG GCT GAT GAG AAA | | miR34a HG R | GTT CAC TGG CCT CAA AGT TGG CAT | | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TGC GGA TGT CCA CGT CA | | U48 F | AGT GAT GAT GAC CCC AGG TA | | U48 R | GGT CAG AGC GCT GCG GTG AT |
Supplementary Figure 2c
| name | sequence | | ----------------- |:--------------------------------:| | miR34a AS F12 | AAA CAC AAG CGT TTA CCT GGG TGC | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT | | miR34a AS R2 | ATA GGT TCA TTT GCC CGA TGT GCC | | miR34a AS R3 | CCA CAG CTG TTG CTT CTG AAT GCT | | miR34a AS Ex3 R1 | TGA TGG CCG CAA CTA ATG ACG GAT |
Supplementary Figure 3c
| name | sequence | | ------------------ |:-------------------------------:| | Luc setII F | AAG ATT CAA AGT GCG CTG CTG | | Luc setII R | TTG CCT GAT ACC TGG CAG ATG | | Renilla pBiDir F1 | TAA CGC GGC CTC TTC TTA TTT | | Renilla pBiDir R1 | GAT TTG CCT GAT TTG CCC ATA | | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
Supplementary Figure 4a
Cloning primers
| name | sequence | | ----------------------- |:--------------------------------:| | miR34aAS cloning F4 | ACG CGT CTC TCC AGC CCG GGA T | | miR34aAS cloning Ex3_1 | AAT GAT GGC CGC AAC TAA TGA CGG |
QPCR primers
| name | sequence | | ------------- |:--------------------------------:| | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TCG GGA TGT CCA CGT CA | | miR34a AS F1 | AGC GGC ATC TCC TCC ACC TGA AA | | miR34a AS R1 | TTG CCT CGT GAG TCC AAG GAG AAT |
Supplementary Figure 4c
| name | sequence | | ------------ |:-------------------------------:| | CCND1 Fwd | CGT GGC CTC TAA GAT GAA GG | | CCND1 Rev | CTG GCA TTT TGG AGA GGA AG | | ß-actin Fwd | AGG TCA TCA CCA TTG GCA ATG AG | | ß-actin Rev | CTT TGC GGA TGT CCA CGT CA |
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