create.SpaCET.object | R Documentation |
Read an ST dataset to create an SpaCET object.
create.SpaCET.object(counts, spotCoordinates, imagePath, platform)
counts |
Count matrix with gene name (row) x spot ID (column). |
spotCoordinates |
Spot coordinate matrix with spot ID (row) x coordinates (column). This matrix should include two columns, i,e., X and Y coordinates, respectively, which represent the position of spots in H&E image. |
imagePath |
Path to the H&E image file. Can be NA if it is not available. |
platform |
A character string indicating the platform, i.e., "Visium", "OldST", or "Slide-Seq". "OldST" is the early in situ capturing method from which "Visium" was developed. |
To create an SpaCET object, user need to input four parameters, i.e., "counts", "spotCoordinates", "imagePath", and "platform". However, if analyzing the Visium data, it is more easy to use 'create.SpaCET.object.10X' to read ST data.
An SpaCET object.
SpaCET_obj <- create.SpaCET.object(counts, spotCoordinates, imagePath, platform)
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