DosageOfGivenCombo: Find the Dosage Given a Subset of Controls

In dsherma7/PolyploidDosageCalling: Calls the Dosage From Tetraploid qPCR Data (CT/RQ Values)

Description

This function determines the calibration sample based on the provided subset of controls. It then computes the RQ values with that samples delta CT value and finds the dosage from those.

Usage

DosageOfGivenCombo(AllData, Controls, Results.Only = FALSE,
  Target = "Assay1 CN ", Reference = "PEF CN", Num.Iters = 5)

Arguments

AllData	A two-dimensional list seperated first by Jobs, then by Plates, containing the data for each experiment.
Controls	data.frame containing the Control values for this experiment. Must have column names of Actual.Name, Name, Dosage; where Actual.Name is what is found exactly in the data sets, Name is the desired output name (often the same as Actual.Name), and Dosage is the Dosage for each control.
Results.Only	FALSE means that the AllData is actually a three-dimensonal list with the third dimension being each sheet in the data sets. If AllData only contains the "Results' section then set this to TRUE.
Target	The reference gene for calling the dosage. Must follow the exact name in datasets.
Num.Iters	The number of iterations to run on the dosage calling algorithm. Each iteration refines the "by" variable by 10. See ManualCall.Trials for more information.
Target	The target gene for calling the dosage. Must follow the exact name in datasets.

dsherma7/PolyploidDosageCalling documentation built on May 23, 2019, 6:06 p.m.