GetDosageForSinglePlate: Linear Model on a Single Plate

In dsherma7/PolyploidDosageCalling: Calls the Dosage From Tetraploid qPCR Data (CT/RQ Values)

Description

This function performs a linear fit of log2(Dosage) as a function of delta CT for a single plate. It also computes a Multinomial distribution of Dosage ~ f(P(RQ)) where P is a 2nd degree polynomial.

Usage

GetDosageForSinglePlate(Plate, job = NA, plate = NA, Controls = NULL,
  MeanRQ = NULL, Target = "Assay1")

Arguments

Plate	A serial data frame of all the relevant values including Job, Plate, RQ, CT, delta CT, and Sample Name for a single plate.
job	Name of the job that this plate is under.
plate	Name of the plate.
Controls	data.frame containing the Control values for this experiment. Must have column names of Actual.Name, Name, Dosage; where Actual.Name is what is found exactly in the data sets, Name is the desired output name (often the same as Actual.Name), and Dosage is the Dosage for each control.
MeanRQ	The Mean RQ value across all Jobs/Plates. Used for scaling the data to provide a more balanced dosage call. Default=NULL means no scaling.

dsherma7/PolyploidDosageCalling documentation built on May 23, 2019, 6:06 p.m.