GetDosage: Linear Regression on PCR Variables

In dsherma7/PolyploidDosageCalling: Calls the Dosage From Tetraploid qPCR Data (CT/RQ Values)

Description

Uses a regression technique to model log(Dosage) as a function of delta CT values.

Usage

GetDosage(AllData, index = 1:length(AllData), Controls = NULL,
  Target = "Assay1")

Arguments

AllData	A serial data frame of all the relevant values including Job, Plate, RQ, CT, delta CT, and Sample Name.
Controls	data.frame containing the Control values for this experiment. Must have column names of Actual.Name, Name, Dosage; where Actual.Name is what is found exactly in the data sets, Name is the desired output name (often the same as Actual.Name), and Dosage is the Dosage for each control.
Results.Only	FALSE means that the AllData is actually a three-dimensonal list with the third dimension being each sheet in the data sets. If AllData only contains the "Results' section then set this to TRUE.
Index	The range of the rows to be used in the fit of the AllData data frame.

dsherma7/PolyploidDosageCalling documentation built on May 23, 2019, 6:06 p.m.