KY_Library_v1.0: Genome-wide annotation for the Sanger sgRNA Library v1.0

In francescojm/CRISPRcleanR: Unsupervised correction of gene independent cell responses to CRISPR-cas9 targeting

Genome-wide annotation for the Sanger sgRNA Library v1.0

Description

A data frame with a named row for each sgRNA of the Sanger sgRNA library presented in [1] including annotations such as targeted genes, and genomic coordinates.

Usage

data(KY_Library_v1.0)

Format

A a row named data frame with 90709 observations (one for each sgRNA) of the following 7 variables.

CODE

alphanumerical identifier of the sgRNAs;

GENES

targeted gene;

EXONE

exone of the targeted genomic region (string with 'ex' prefix followed by the exone number);

CHRM

chromosome of where the targeted region resides (string)

STRAND

targeted DNA strand ('+' or '-')

STARTpos

starting genomic coordinate of the targeted genomic region (numeric);

ENDpos

ending genomic coordinate of the targeted genomic region (numeric).

seq

nucelotidic sequence of the sgRNAs without the PAM. (string).

References

[1] Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Onishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJP, Iorio F, Pina C, Vassiliou GS, Yusa K. A CRISPR dropout screen identifies genetic vulnerabilities and therapeutic targets in acute myeloid leukaemia. Cell Reports 2016 Oct 18;17(4):1193-1205

Examples

data(KY_Library_v1.0)
head(KY_Library_v1.0)

francescojm/CRISPRcleanR documentation built on April 30, 2023, 5:41 a.m.