HermesData-class: 'HermesData' and 'RangedHermesData'

In insightsengineering/hermes: Preprocessing, analyzing, and reporting of RNA-seq data

HermesData and RangedHermesData

Description

The HermesData class is an extension of SummarizedExperiment::SummarizedExperiment with additional validation criteria.

Usage

HermesData(object)

HermesDataFromMatrix(counts, ...)

Arguments

object	(SummarizedExperiment) input to create the HermesData object from. If this is a RangedSummarizedExperiment, then the result will be RangedHermesData.
counts	(matrix) counts to create the HermesData object from.
...	additional arguments, e.g. rowData, colData, etc. passed to SummarizedExperiment::SummarizedExperiment() internally. Note that if rowRanges is passed instead of rowData, then the result will be a RangedHermesData object.

Details

The additional criteria are:

• The first assay must be counts containing non-missing, integer, non-negative values. Note that rename() can be used to edit the assay name to counts if needed.

• The following columns must be in rowData:

  • symbol (also often called HGNC or similar, example: "INMT")

  • desc (the gene name, example: "indolethylamine N-methyltransferase")

  • chromosome (the chromosome as string, example: "7")

  • size (the size of the gene in base pairs, e.g 5468)

  • low_expression_flag (can be populated with add_quality_flags())

• The following columns must be in colData:

  • low_depth_flag (can be populated with add_quality_flags())

  • tech_failure_flag (can be populated with add_quality_flags())

• The object must have unique row and column names. The row names are the gene names and the column names are the sample names.

Analogously, RangedHermesData is an extension of SummarizedExperiment::RangedSummarizedExperiment and has the same additional validation requirements. Methods can be defined for both classes at the same time with the AnyHermesData signature.

A Biobase::ExpressionSet object can be imported by using the SummarizedExperiment::makeSummarizedExperimentFromExpressionSet() function to first convert it to a SummarizedExperiment::SummarizedExperiment object before converting it again into a HermesData object.

Value

An object of class AnyHermesData (HermesData or RangedHermesData).

Slots

prefix

common prefix of the gene IDs (row names).

Note

• Note that we use S4Vectors::setValidity2() to define the validity method, which allows us to turn off the validity checks in internal functions where intermediate objects may not be valid within the scope of the function.

• It can be helpful to convert character and logical variables to factors in colData() (before or after the HermesData creation). We provide the utility function df_cols_to_factor() to simplify this task, but leave it to the user to allow for full control of the details.

See Also

rename() for renaming columns of the input data.

Examples

# Convert an `ExpressionSet` to a `RangedSummarizedExperiment`.
ranged_summarized_experiment <- makeSummarizedExperimentFromExpressionSet(expression_set)

# Then convert to `RangedHermesData`.
HermesData(ranged_summarized_experiment)

# Create objects starting from a `SummarizedExperiment`.
hermes_data <- HermesData(summarized_experiment)
hermes_data

# Create objects from a matrix. Note that additional arguments are not required but possible.
counts_matrix <- assay(summarized_experiment)
counts_hermes_data <- HermesDataFromMatrix(counts_matrix)

insightsengineering/hermes documentation built on Dec. 15, 2024, 8:07 a.m.