DoubletDetection_with_scrublet: DoubletDetection_with_scrublet

View source: R/SingleCellRNASeq.R

DoubletDetection_with_scrubletR Documentation

DoubletDetection_with_scrublet

Description

DoubletDetection_with_scrublet

Usage

DoubletDetection_with_scrublet(
  object,
  expected.doublet.rate = 0.03,
  seed = 2021L,
  PCs = 30L,
  min.cells = 10,
  min_gene_variablity = 85
)

Arguments

object

The SingCellaR object.

expected.doublet.rate

The expected fraction of transcriptomes that are doublets, typically 0.05-0.1.

seed

The seed number

PCs

The number of input PCA components

min.cells

Used for gene filtering prior to PCA. Genes expressed at fewer than min_counts, default=3, in fewer than min_cells are excluded.

min_gene_variablity

Used for gene filtering prior to PCA. Keep the most highly variable genes for the analysis.


supatt-lab/SingCellaR documentation built on Aug. 24, 2023, 5:49 p.m.