last: lastal wrapper

In CNEr: CNE Detection and Visualization

Description

Wrapper function of lastal to do the pairwise whole genome alignment. This function doesn't work on Windows platform.

Usage

  lastal(db, queryFn,
         outputFn=sub("\\.(fa|fasta)$", ".maf", 
                      paste(basename(db), basename(queryFn), sep = ","),
                      ignore.case = TRUE),
         distance=c("far", "medium", "near"), binary="lastal",
         mc.cores=getOption("mc.cores", 2L), echoCommand=FALSE)

Arguments

db	character(1): the file name of target assembly's lastal index.
queryFn	character(1): the file name of query assembly fasta file.
outputFn	character(1): the file name of the output maf file.
distance	It can be "far", "medium" or "near". It decides the score matrix used in lastz aligner. See '?scoringMatrix' for more details.
binary	character(1): the name/filename of the binary lastal to call.
mc.cores	integer(1): the number of threads to use. By default, getOption("mc.cores", 2L).
echoCommand	boolean(1): When TRUE, only the command to run lastal is returned.

Value

A character(1) vector of ouput maf file names.

Note

lastal aligner must be installed on the machine to use this function.

Author(s)

Ge Tan

References

http://last.cbrc.jp/

See Also

lastz

Examples

  ## Not run: 
    assemblyDir <- "/Users/gtan/OneDrive/Project/CSC/CNEr/2bit"
    ## Build the lastdb index
    system2(command="lastdb", args=c("-c", file.path(assemblyDir, "danRer10"),
                                     file.path(assemblyDir, "danRer10.fa")))

    ## Run lastal aligner
    lastal(db=file.path(assemblyDir, "danRer10"),
         queryFn=file.path(assemblyDir, "hg38.fa"),
         outputFn=file.path(axtDir, "danRer10.hg38.maf"),
         distance="far", binary="lastal", mc.cores=4L)

    ## maf to psl 
    psls <- file.path(axtDir, "danRer10.hg38.psl")
    system2(command="maf-convert", 
            args=c("psl", file.path(axtDir, "danRer10.hg38.maf"),
                   ">", psls))
  
## End(Not run)

CNEr documentation built on Nov. 8, 2020, 5:36 p.m.