checkPrimer: checkPrimer

Description Usage Arguments Value Author(s) Examples

View source: R/primerpcr.R

Description

checkPrimer

Usage

1
checkPrimer(pp, genome, roi = NULL)

Arguments

pp

data.frame defining primers, or output of callPrimer3. minimal columns = PRIMER_LEFT_SEQUENCE,PRIMER_RIGHT_SEQUENCE

genome

BSgenome object

roi

makeROI object

Value

list of GRanges with primer locations

Author(s)

Diana Low

Examples

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# create a primer pair
roi
primer_pair <- data.frame(PRIMER_LEFT_SEQUENCE="agctcttgaaattggagctgac",
                          PRIMER_RIGHT_SEQUENCE="cttagaaagaacaggaaatcc",
                          stringsAsFactors=FALSE)

SPLINTER documentation built on May 31, 2017, 2:54 p.m.