dada2-package: DADA2 package

In dada2: Accurate, high-resolution sample inference from amplicon sequencing data

Description

The dada2 package is centered around the DADA2 algorithm for accurate high-resolution of sample composition from amplicon sequencing data. The DADA2 algorithm is both more sensitive and more specific than commonly used OTU methods, and resolves amplicon sequence variants (ASVs) that differ by as little as one nucleotide.

Details

The dada2 package also provides a full set of tools for taking raw amplicon sequencing data all the way through to a feature table representing sample composition. Provided facilities include:

• Quality filtering (filterAndTrim, fastqFilter, fastqPairedFilter)

• Dereplication (derepFastq)

• Learn error rates (learnErrors)

• Sample Inference (dada)

• Chimera Removal (removeBimeraDenovo, isBimeraDenovo, isBimeraDenovoTable)

• Merging of Paired Reads (mergePairs)

• Taxonomic Classification (assignTaxonomy, assignSpecies)

Author(s)

Benjamin Callahan [e-mail]

Paul J McMurdie II [e-mail]

Michael Rosen [e-mail]

Susan Holmes [e-mail]

dada2 documentation built on Nov. 8, 2020, 6:48 p.m.