cubeTGD: Inheritance Cube: tGD

In MGDrivE: Mosquito Gene Drive Explorer

Description

The trans-complementing Gene Drive (tGD) is a 1-locus, 2 target site drive. The first target site corresponds to the Cas protein, the second to an effector gene and the gRNAs. There are two sets of gRNAs, because each target site may have different cutting/homing/resistance rates, and each sex can have different rates for all of those things. Additionally, the parent that you receive your Cas from dictates its efficiency. Therefor, this construct has 5 alleles at the first locus and 4 alleles at the second.

• Locus 1

  • W: Wild-type

  • P: Paternal Cas9

  • M: Maternal Cas9

  • R: Resistant allele 1

  • B: Resistant allele 2

• Locus 2

  • W: Wild-type

  • G: gRNAs

  • R: Resistant 1

  • B: Resistant 2

Usage

cubeTGD(
  cM1 = 0,
  cM2 = 0,
  cP1 = 0,
  cP2 = 0,
  hM1 = 0,
  hM2 = 0,
  hP1 = 0,
  hP2 = 0,
  rM1 = 0,
  rM2 = 0,
  rP1 = 0,
  rP2 = 0,
  crM = 0,
  crP = 0,
  eta = NULL,
  phi = NULL,
  omega = NULL,
  xiF = NULL,
  xiM = NULL,
  s = NULL
)

Arguments

cM1	Maternally inherited Cas9 cutting rate at locus 1
cM2	Maternally inherited Cas9 cutting rate at locus 2
cP1	Paternally inherited Cas9 cutting rate at locus 1
cP2	Paternally inherited Cas9 cutting rate at locus 2
hM1	Maternally inherited Cas9 homing efficiency at locus 1
hM2	Maternally inherited Cas9 homing efficiency at locus 2
hP1	Paternally inherited Cas9 homing efficiency at locus 1
hP2	Paternally inherited Cas9 homing efficiency at locus 2
rM1	Maternally inherited Cas9 resistance efficiency at locus 1
rM2	Maternally inherited Cas9 resistance efficiency at locus 2
rP1	Paternally inherited Cas9 resistance efficiency at locus 1
rP2	Paternally inherited Cas9 resistance efficiency at locus 2
crM	Maternal crossover rate, 0 is completely linked, 0.5 is unlinked, 1.0 is complete divergence
crP	Paternal crossover rate, 0 is completely linked, 0.5 is unlinked, 1.0 is complete divergence
eta	Genotype-specific mating fitness
phi	Genotype-specific sex ratio at emergence
omega	Genotype-specific multiplicative modifier of adult mortality
xiF	Genotype-specific female pupatory success
xiM	Genotype-specific male pupatory success
s	Genotype-specific fractional reduction(increase) in fertility

Details

This drive corresponds to the transcomplementing gene drive developed by the Gantz and Bier lab.

Value

Named list containing the inheritance cube, transition matrix, genotypes, wild-type allele, and all genotype-specific parameters.

MGDrivE documentation built on Oct. 23, 2020, 7:28 p.m.