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#'
#' Plot peptide modification frequencies
#'
#' The input is a data.frame, as obtained from modsToTableByRaw().
#'
#'
#'
#' @param tbl A data.frame with 'fc.raw.file', 'modification_names' (can be a factor), and 'Freq' (0-100)
#' @param y_max The upper limit of the y-axis's (==Freq); useful for multiple plots with identical limits; if 'NA' the limit is computed from the given 'tbl'
#' @param show_missing_modification_levels If 'tbl$modification_names' is a factor and has more (but missing) levels than actually used, should missing values be dropped or assumed as '0' frequency?
#' @return GGplot object
#'
#' @import ggplot2
#' @export
#'
#' @examples
#'
#' data = data.frame(fc.raw.file = rep(c("file A", "file B"), each=3),
#' modifications = c("Oxidation (M)",
#' "Unmodified",
#' "Oxidation (M), Acetyl (Protein N-term)",
#' "2 Oxidation (M)",
#' "Unmodified",
#' "Unmodified"))
#' tbl = modsToTableByRaw(data)
#' plot_peptideMods(tbl,show_missing_modification_levels = TRUE)
#'
plot_peptideMods = function(tbl, y_max = NA, show_missing_modification_levels = TRUE)
{
if (is.na(y_max)){
y_max = max(tbl$Freq, na.rm = TRUE)
}
## augment '0'-frequency for missing factors
if (show_missing_modification_levels) {
tbl$fc.raw.file = droplevels(as.factor(tbl$fc.raw.file)) ## we need fc.raw.file to be a factor, but we do not want its unused levels
all_combinations = expand.grid(lapply(tbl[c("fc.raw.file","modification_names")], levels))
new_tbl = merge(tbl, all_combinations , all.y=TRUE)
new_tbl$Freq[is.na(new_tbl$Freq)] = 0 ## replace new 'NA's with 0
tbl = new_tbl
}
p = ggplot(tbl, aes_string(x = "fc.raw.file", y = "Freq", fill = "modification_names", colour="modification_names")) + ## use 'colour' for outline of '0'-frequency features (invisible otherwise)
geom_col(position = "dodge") +
xlab("") +
ylab("Occurence [%]") +
scale_x_discrete_reverse(tbl$fc.raw.file) +
guides(fill = guide_legend(reverse = TRUE), color = guide_none()) + ## reverse order of mods in legend, to match order in plot
ylim(0, y_max) +
ggtitle("EVD: variable modifications per Raw file") +
coord_flip()
return(p)
}
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