ScreenR-package: Tools for analyzing shRNAs screening data
In EmanuelSoda/ScreenR: Package to Perform High Throughput Biological Screening

ScreenR-package

R Documentation

Tools for analyzing shRNAs screening data

Description

ScreenR is an easy and effective package to perform hits identification in loss of function High Throughput Biological Screening performed with shRNAs library. ScreenR combines the power of software like edgeR with the simplicity of the Tidyverse metapackage. ScreenR executes a pipeline able to find candidate hits from barcode counts data and integrates a wide range of visualization for each step of the analysis.

Details

ScreenR takes the a count table as input and create the screenr_object to perform the analysis. Throught the pipeline ScreenR enable the user to perform quality control, visual inspection, dimensionality reduction of the data. Using three statistical methods:

it is able to find new candidate hits. Moreover in order to improve the quality of the hit found it is also possible to further filter the list of hit using other filter like the variance and the slope filters.