plot_barcode_lost_for_gene: Plot number of barcode lost for gene

In EmanuelSoda/ScreenR: Package to Perform High Throughput Biological Screening

Plot number of barcode lost for gene

Description

This function plots the number of barcodes lost in each sample for each gene. Usually in a genetic screening each gene is is associated with multiple shRNAs and so barcodes. For this reason a reasonable number of barcodes associated with the gene has to be retrieved in order to have a robust result. Visualizing the number of genes that have lost lot's of barcode is a Quality Check procedure in order to be aware of the number of barcode for the hit identified.

Usage

plot_barcode_lost_for_gene(screenR_Object, facet = TRUE, samples)

Arguments

screenR_Object	The ScreenR object obtained using the create_screenr_object
facet	A boolean to use the facet.
samples	A vector of samples that as to be visualize

Value

Return the plot displaying the number of barcode lost for each gene in each sample.

Examples

object <- get0("object", envir = asNamespace("ScreenR"))

plot_barcode_lost_for_gene(object,
    samples = c("Time3_A", "Time3_B")
)
plot_barcode_lost_for_gene(object,
    samples = c("Time3_A", "Time3_B"),
    facet = FALSE
)

EmanuelSoda/ScreenR documentation built on Sept. 29, 2023, 12:33 a.m.