extract_proximal_4bases_PAM: extract_proximal_4bases_PAM

Description Usage Arguments Value Author(s) References

View source: R/extract_proximal_4bases_PAM.R

Description

Extracts the proximal 4 bases upstream of the PAM in a sgRNA library data frame into "GCC", "TT", or "Other" (Graf et al. 2019).

Usage

1

Arguments

library

A data frame containing the library file in which the first column gives the sgRNA sequence and the second column gives the sgRNA ID.

Value

A data frame with a column named 'proximal_4bases_pam'.

Author(s)

Alex T. Kalinka, alex.kalinka@cancer.org.uk

References

Graf, R. et al. (2019) sgRNA sequence motifs blocking efficient CRISPR/Cas9-mediated gene editing. Cell Rep 26: 1098-1103.


alex-kalinka-cruk/fgcQC documentation built on June 23, 2020, 9:05 p.m.